Anacardic acid as an inhibitor of glyceraldehyde triphosphate dehydrogenase in echinococcosis and as a drug for echinococcosis

Abstract

The invention discloses applications of anacardic acid as an inhibitor of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of hydatid cysts and a medicine for treating echinococcosis. According to the invention, the applications of anacardic acid as the inhibitor of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the hydatid cysts and the medicine for treating echinococcosis are disclosed for the first time, so that the applications of anacardic acid are expanded, and a novel medicine for treating echinococcosis is developed.

Description

technical field

[0001] The invention relates to the application of a newborn Chinese herbal medicine monomer anacardic acid in the fields of inhibiting the activity of glyceraldehyde triphosphate dehydrogenase and treating echinococcosis. Background technique

[0002] Echinococcosis is a serious parasitic disease caused by Echinococcus parasitizing in humans or host animals. my country is a high-incidence area for both cystic and vesicular echinococcosis. At present, the most widely used anti-echinococcosis drug in clinical practice is albendazole, but the intestinal absorption rate of this drug is very poor, and it generally can only inhibit the growth of parasites but cannot completely and effectively kill parasites, so patients must be treated for a long time. Take this medicine in large quantities to achieve therapeutic effect. At the same time, a large number of studies have found that the drug can cause serious adverse drug reactions in multiple systems. Therefore, ...

Examples

Embodiment 1

[0029] Example 1: Effect of Anacardic Acid on the Enzyme Activity of Echinococcus Glyceraldehyde Triphosphate Dehydrogenase

[0030] Purpose and principle: In order to verify that anacardic acid can inhibit glyceraldehyde triphosphate dehydrogenase in Echinococcus, the principle of GAPDH enzyme activity detection is glyceraldehyde 3-phosphate+H3PO4+NAD+↔1,3-glycerol diphosphate+NADH+H+. As NADH is produced in the reaction mixture, its absorbance at 340 nm increases. Therefore, the inhibitory effect of anacardic acid on GAPDH can be judged according to the 340nm OD value recorded in the reaction between different concentrations of anacardic acid and GAPDH.

[0031] Method: Reagents included 0.01mol / L sodium pyrophosphate, 0.02 mol / L sodium phosphate, 0.25 mmol / L NAD, 3 μmol / L DTT, 0.25 mmol / L 3-phosphoglyceraldehyde (D-G3P), 5 μg purified GAPDH, the reaction temperature is 25°C. Record the OD value at 340nm wavelength for 0-5min to obtain the catalytic activity of GAPDH. The...

Embodiment 2

[0034] Example 2: Effect of anacardic acid on Echinococcus granulosus

[0035] Purpose and principle: To observe the survival rate of protoscoleum treated with drugs in vitro by trypan blue staining method, so as to observe the effect of drugs on Echinococcus granulosus. Normal living cells have a complete membrane structure, which can repel trypan blue and prevent it from entering the cell; while cells with inactive or incomplete membranes have increased membrane permeability and can be stained blue by trypan blue color. It is generally considered that the integrity of the cell membrane is lost, that is, it is considered dead.

[0036] Method: The cleaned protoscole of Echinococcus granulosus was added to the 24-well plate, and the control group and drug-dosed group were set up. The control group was added with solvent, and the drug-dosed group was added with anacardic acid with different concentrations. Pan blue staining was used to record the survival rate of the protosco...

Embodiment 3

[0039] Example 3: Effect of anacardic acid on alveolar echinococcosis

[0040] Purpose and principle: The effect of anacardic acid on alveolar echinococcosis was determined by alkaline phosphatase. The determination experiment of alkaline phosphatase must use the indirect measurement of the content of alkaline phosphatase to reflect the damage degree of alveolar Echinococcus tissue. The detection principle is that alkaline phosphatase will be released into the supernatant when the alveolar Echinococcus tissue is damaged, but not released when it is not damaged, so the degree of damage is directly proportional to alkaline phosphatase.

[0041] Method: Add the vesicles of the cultured alveolar coccus tissue into the 24-well plate, set up the control group and the dosing group, add the solvent in the control group, add anacardic acid with different concentrations in the dosing group, and incubate on the 3rd and 7th day At the time, the supernatant was aspirated, and the content ...

technical field

[0001] The invention relates to the application of a newborn Chinese herbal medicine monomer anacardic acid in the fields of inhibiting the activity of glyceraldehyde triphosphate dehydrogenase and treating echinococcosis. Background technique

[0002] Echinococcosis is a serious parasitic disease caused by Echinococcus parasitizing in humans or host animals. my country is a high-incidence area for both cystic and vesicular echinococcosis. At present, the most widely used anti-echinococcosis drug in clinical practice is albendazole, but the intestinal absorption rate of this drug is very poor, and it generally can only inhibit the growth of parasites but cannot completely and effectively kill parasites, so patients must be treated for a long time. Take this medicine in large quantities to achieve therapeutic effect. At the same time, a large number of studies have found that the drug can cause serious adverse drug reactions in multiple systems. Therefore, ...

Examples

Embodiment 1

[0029] Example 1: Effect of Anacardic Acid on the Enzyme Activity of Echinococcus Glyceraldehyde Triphosphate Dehydrogenase

[0030] Purpose and principle: In order to verify that anacardic acid can inhibit glyceraldehyde triphosphate dehydrogenase in Echinococcus, the principle of GAPDH enzyme activity detection is glyceraldehyde 3-phosphate+H3PO4+NAD+↔1,3-glycerol diphosphate+NADH+H+. As NADH is produced in the reaction mixture, its absorbance at 340 nm increases. Therefore, the inhibitory effect of anacardic acid on GAPDH can be judged according to the 340nm OD value recorded in the reaction between different concentrations of anacardic acid and GAPDH.

[0031] Method: Reagents included 0.01mol / L sodium pyrophosphate, 0.02 mol / L sodium phosphate, 0.25 mmol / L NAD, 3 μmol / L DTT, 0.25 mmol / L 3-phosphoglyceraldehyde (D-G3P), 5 μg purified GAPDH, the reaction temperature is 25°C. Record the OD value at 340nm wavelength for 0-5min to obtain the catalytic activity of GAPDH. The...

Embodiment 2

[0034] Example 2: Effect of anacardic acid on Echinococcus granulosus

[0035] Purpose and principle: To observe the survival rate of protoscoleum treated with drugs in vitro by trypan blue staining method, so as to observe the effect of drugs on Echinococcus granulosus. Normal living cells have a complete membrane structure, which can repel trypan blue and prevent it from entering the cell; while cells with inactive or incomplete membranes have increased membrane permeability and can be stained blue by trypan blue color. It is generally considered that the integrity of the cell membrane is lost, that is, it is considered dead.

[0036] Method: The cleaned protoscole of Echinococcus granulosus was added to the 24-well plate, and the control group and drug-dosed group were set up. The control group was added with solvent, and the drug-dosed group was added with anacardic acid with different concentrations. Pan blue staining was used to record the survival rate of the protosco...

Embodiment 3

[0039] Example 3: Effect of anacardic acid on alveolar echinococcosis

[0040] Purpose and principle: The effect of anacardic acid on alveolar echinococcosis was determined by alkaline phosphatase. The determination experiment of alkaline phosphatase must use the indirect measurement of the content of alkaline phosphatase to reflect the damage degree of alveolar Echinococcus tissue. The detection principle is that alkaline phosphatase will be released into the supernatant when the alveolar Echinococcus tissue is damaged, but not released when it is not damaged, so the degree of damage is directly proportional to alkaline phosphatase.

[0041] Method: Add the vesicles of the cultured alveolar coccus tissue into the 24-well plate, set up the control group and the dosing group, add the solvent in the control group, add anacardic acid with different concentrations in the dosing group, and incubate on the 3rd and 7th day At the time, the supernatant was aspirated, and the content ...

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