Probiotic composition with anti-tumor function as well as preparation method and application thereof
An anti-tumor and probiotic technology, applied in the field of biomedicine, can solve problems such as physical injury, side effects, not cancer prevention, etc., and achieve the effect of high safety, excellent efficacy and avoiding drug loss.
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Embodiment 1
[0031] 1) Prepare probiotic dry powder by selecting materials:
[0032] 1) Inoculate Lactobacillus rhamnosus, Lactobacillus casei, and Bifidobacterium longum in the fermentation medium after being activated in the culture medium, and culture with shaking, so that the concentration of the bacteria reaches the logarithmic growth phase of 0.5 to 1.0×10 9 cfu / ml;
[0033] Preparation of Bifidobacterium seed solution: Take out the strain preservation tube, use Bifidobacterium solid medium to scratch the plate for recovery, and culture anaerobically at 37 degrees for 72 hours. Pick a single colony under the plate and inoculate it in 50 ml of bifidobacterium medium, and culture it anaerobically at 37 degrees in an incubator for 48 hours. The seeds are inoculated into a 10L large triangular flask with an inoculum volume of 5% (the liquid volume is 9.5L), cultured anaerobically at 37 degrees for 20-28 hours, and the concentration of the bacteria liquid is detected. The bacteria conten...
Embodiment 2
[0043] The specific steps are the same as in Example 1, the difference being that: ingredients in step (3): Weigh and sieve the raw materials according to 60% of probiotic dry powder, 25% of astragalus extract powder, and 15% of asparagus extract powder, The above contents are all percentages by weight.
Embodiment 3
[0045] The specific steps are the same as in Example 1, the difference being: ingredients in step (3): Weigh and sieve raw materials, mix according to 60% of probiotic dry powder, 20% of astragalus extract powder, and 20% of asparagus extract powder, above The contents are all percentages by weight.
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