Unlock instant, AI-driven research and patent intelligence for your innovation.
A flow cytometer excitation light source system and calibration method
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A flow cytometer and excitation light source technology, applied in the field of flow cytometer excitation light source system and calibration, can solve problems such as poor alignment accuracy, achieve the effects of improving accuracy, overcoming the deviation of light spots, and realizing automatic calibration
Active Publication Date: 2018-09-18
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
View PDF9 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defect of poor alignment accuracy between the spot center and the target in the prior art
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0045] This embodiment provides a flow cytometer excitation light source system, such as figure 1 As shown, the system 10 includes:
[0046] The light source 1 is used to emit excitation light; preferably, the light source 1 can be a 532nm laser light source with a divergence angle of 1.5mrad and a spot diameter of 1mm.
[0047] The adjustment device 2 is connected with the feedback control device 4, and is used to receive the excitation light emitted by the light source 1 and adjust the propagation direction of the excitation light according to the control signal from the feedback control device 4 before emitting; the adjustment direction can be 360 degrees around the optical axis The direction, preferably, can be two groups of directions, up and down and left and right, for adjusting the excitation beam.
[0048] The spectroscopic and detection device 3 is connected with the feedback control device 4, and is used to divide the light emitted by the adjustment device 2 into...
Embodiment 2
[0060] This embodiment provides a calibration method for the excitation light source system of the flow cytometer in the above embodiment 1, for example, the method is applied to a feedback control device, such as Figure 4 As shown, the method includes the following steps:
[0061] S1, the feedback control device 4 receives the signal transmitted by the spectroscopic and detection device 3 and includes the current light intensity I and spot position (x, y) information;
[0062] S2. The feedback control device 4 judges whether the distance value r is less than a preset value, wherein, r= (x 0 ,y 0 ) represents the central position of the light spot detected by the spectroscopic and detection device 3 when the light emitted by the shaping and focusing device 5 is focused and irradiated on the target; when the distance value r is less than the preset value, enter step S3; when the distance value r is greater than or equal to the preset value, go to step S4. Preferably, the ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
A flow cytometer excitation light source system and a correction method are disclosed. The system comprises a light source, an adjusting device, a light splitting and detecting device, a shaping and focusing device and a feedback control device, wherein the adjusting device is connected to the feedback control device and used for receiving excitation light emitted from the light source, adjusting the propagation direction of the excitation light according to control signals from the feedback control device and then allowing the light to emerge; the light splitting and detecting device is connected to the feedback control device and used for splitting light emergent from the adjusting device into two beams, detecting light intensity and light spot positions of one of the two beams, transmitting signals including information of the light intensity and the light spot positions to the feedback control device and allowing the other beam to emerge; the shaping and focusing device is used for shaping and focusing the light emergent from the light splitting and detecting device and allowing the light to emerge to an object; and the feedback control device is used for receiving signals including the information of the light intensity and the light spot positions with the signals being transmitted by the light splitting and detecting device, calculating an adjusting quantity and a light intensity correction according to the signals, and transmitting control signals containing the adjusting quantity to the adjusting device. The system and the method are advantaged by high alignment precision.
Description
technical field [0001] The invention relates to the field of optical measuring instruments, in particular to an excitation light source system and a calibration method for a flow cytometer. Background technique [0002] Flow cytometry is an instrument that can quickly analyze the characteristics (such as size, refractive index, complexity of internal structure, etc.) of cells or some special particles (such as polystyrene microspheres). The sample containing cells is compressed and focused by the sheath fluid and enters the fluid pool to form a laminar flow. The cells are compressed on the sample streamline and pass through the laser spot one by one. Use the detector in front of the optical axis and at 90° to the optical axis. Direction test The scattered light generated by the cells passing through the laser spot and the specific fluorescence generated by the fluorescent dyes carried by the cells are used to test some characteristics of the cells. [0003] Flow cytometry r...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14G01J1/08G01J1/04
CPCG01J1/0422G01J1/08G01J2001/086G01N15/14
Inventor 陈忠祥王策吴云良裴智果钟金凤严心涛武晓东
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Login to View More 
Login to View More