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Immunopotentiator and its preparation method and application

An immune enhancer and oral vaccine technology, applied in the field of immune enhancer and its preparation, can solve the problems of low neutralizing antibody titer, poor immunogenicity, animal disease and the like

Active Publication Date: 2019-10-15
北京中联康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional live attenuated vaccines may cause disease in vaccinated animals due to residual virulence or in vivo reproduction variation, which has certain risks
At present, dogs are immunized orally, and it has been confirmed that dogs can be induced to produce rabies virus antibodies to obtain resistance to the virus. However, oral immunization of most existing rabies virus oral vaccine strains is poor in immunogenicity, and the neutralizing antibody titers produced The degree is low, so it is necessary to use a certain immune enhancer to enhance the immune effect

Method used

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  • Immunopotentiator and its preparation method and application
  • Immunopotentiator and its preparation method and application
  • Immunopotentiator and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of Immunopotentiator for Rabies Oral Live Vaccine

[0026] (1) Add 1g of chitosan to 100ml of 0.8% acetic acid solution, mix well, and use 7.5% NaHCO 3 Adjust the pH value of the solution to 7.0, adjust the pH slowly to prevent precipitation, then set the volume to 100ml, pressurize at 121°C for 20min, and prepare a 1% chitosan solution. Mix the high-pressure chitosan solution with the virus solution 1:1, which is the rabies immune enhancer 1.

[0027] (2) Weigh 50 mg of α-mannan peptide and add it into 10 ml of PBS with pH 7.2, mix well, and after fully dissolved, filter to sterilize at 0.22 μm. Take 0.5 ml of 5 mg / ml α-mannan peptide solution and add it to 2 ml of virus liquid, which is the rabies immune enhancer 2.

[0028] (3) Add 100mg of trimethyl quaternary ammonium chitosan (TMC) to 10ml of PBS with a pH value of 7.2, mix well, and after fully dissolving, pressurize at 121°C for 20min, add 1ml of TMC after high pressure to 1ml of virus liquid Amon...

Embodiment 2

[0032] Toxicity Test of Immunopotentiator to Rabies Virus

Embodiment approach

[0034] 1) The virus titer was 10 8.3 TCID 50 The rabies virus / ml is diluted 10 times with serum-containing DMEM medium, that is, 10 -1 , 10 -2 ,·····,10 -9 .

[0035] 2) Mix the immunopotentiator and the virus solution with the rabies virus solution at a ratio of 1:1, and measure the TCID50 of the mixed solution virus after 1 hour at 37°C in a water bath; at the same time, only the immunopotentiator without adding Virus liquid (immunopotentiator mixed with DMEM medium) and only virus fluid without immunopotentiator as a control.

[0036] 2.TCID 50 The measurement results are shown in Table 1.

[0037] Table 1 Virus fluid TCID50 measurement results

[0038] group lgTCID 50 / ml

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Abstract

The invention provides an immunopotentiator and a preparation method and an application thereof, wherein the immunopotentiator comprises at least one of chitosan, N,N,N-trimethyl chitosan quaternary ammonium salt and mannatide. The immunopotentiator can significantly improve immune response of immunized animals, increases the antibody level, thereby improving the immune effect of vaccines, shortening the immune window period and prolonging the protection period.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immune enhancer and its preparation method and application. Background technique [0002] Rabies, also known as mad dog disease or hydrophobia, is a zoonotic infectious disease caused by rabies virus. Rabies mainly affects the central nervous system. Once a person becomes ill, there is no effective clinical treatment. It is the only acute infectious disease with a mortality rate of up to 100%. About 55,000 people die of rabies every year in the world. Since 2003, the annual death toll of rabies in my country has exceeded 2,000. It is the second last in the world, after India. The spread of rabies virus has seriously affected the public health security of our country and has become a very prominent social problem. [0003] The existing vaccines for the prevention of rabies in my country are mainly inactivated vaccines. Inactivated vaccines are mainly made by using spinner bottles...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61K31/722A61P37/04A61K39/205A61P31/14
Inventor 路明华张二磊秦梅李艳侯艳红刘延亭况慧星刘俊生潘强熊炜
Owner 北京中联康生物科技股份有限公司
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