35 results about "N-trimethyl chitosan" patented technology

The invention discloses a preparation method of a trinary composite stent of plasmid DNA / fibrin gel / polymer. The preparation method comprises the following steps of: geometrically and uniformly mixing an N-trimethyl chitosan solution and a plasmid DNA solution to prepare plasmid DNA composite particles; dissolving fibrinogen in physiological saline to prepare a fibrinogen solution; dissolving thrombin in a calcium chloride solution to prepare a thrombin calcium chloride solution; sterilizing a polymer porous stent with ethanol; simultaneously filling the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution with the same volumes in the polymer porous stent by utilizing negative pressure; and arranging the stent filled with the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution in a constant-temperature oven to fully gelatinize the fiber protein. The preparation method is simple and convenient, uses materials with wide resources and can simulate the composition and the structure of the cartilage of a human body. The composite stent of the invention has favorable mechanical property and biological activity, can accelerate the restoration of the cartilage and the osseous tissue and have strong application values of cartilage and bone restoration.

The invention discloses base fertilizer for a water chestnut planting field. The base fertilizer is characterized by being prepared from the following raw materials in percentage by mass: 28 percent to 32 percent of composted cattle pen manure, 8 percent to 12 percent of phosphorus fertilizer, 2 percent to 4 percent of zinc-boron fertilizer, 28 percent to 32 percent of plant ash, 20 percent to 24 percent of oilseed cake and 4 percent to 6 percent of an auxiliary material. The auxiliary material comprises the following components in parts by weight: 14 to 20 parts of modified bamboo powder, 4 to 8 parts of vermiculite powder, 5 to 9 parts of sepiolite powder, 2 to 4 parts of sodium humate, 2 to 4 parts of straw powder and 3 to 5 parts of turf. The modified bamboo powder is prepared by adopting the following process: stirring N,N,N-trimethyl chitosan, glacial acetic acid and water; dropwise adding a silver nitrate solution under a stirring state; then dropwise adding a sodium borohydride solution and stirring to obtain a prefabricated material; stirring gelatin and the water; adding sodium hydroxide and stirring; adjusting the pH (Potential of Hydrogen) value to be 7.1 to 7.5; adding succinic anhydride and stirring; drying and crushing; adding a silane coupling agent, the bamboo powder, the prefabricated material and toluene; raising the temperature, stirring, washing, drying and crushing to obtain the modified bamboo powder.

The invention discloses rapeseed gel oil having a slimming effect. The rapeseed gel oil comprises the following components in parts by weight: 102-103 parts of rapeseed oil, 4-5 parts of yeast glucan, 2-3 parts of yeast vitamins B, 8-9 parts of monostearin stearic acid, 0.35-0.4 part of erucic acid sucrose ester, 2-3 parts of folium cortex eucommiae n-butyl alcohol abstracts, 2.5-3 parts of maltitol, 3-4 parts of N-trimethyl chitosan, 2.2-2.3 parts of gamma-oryzanol, 1.9-2 parts of beta-sitosterol and a proper amount of water. According to the invention, the constructed functional dispersoid, rapeseed oil, folium cortex eucommiae n-butyl alcohol abstracts, maltitol, gelator gamma-oryzanol and beta-sitosterol are prepared into the rapeseed gel oil; the rapeseed gel oil has special slimming effect and has excellent resistance to oxidation; the nutrition of the rapeseed gel oil is enriched; the yeast glucan orally taken together with yeast vitamins B has the slimming effect; the folium cortex eucommiae n-butyl alcohol abstracts have excellent resistance to oxidation for the rapeseed gel oil.

The invention provides an N-trimethyl chitosan microsphere, a preparation method thereof and application of the microsphere in reduction of dielectric loss of vegetable oil, belonging to the field ofmodified chitosan. The preparation method of the N-trimethyl chitosan microsphere comprises the following steps: carrying out hybrid reaction on chitosan with the visocisty of 0.25-0.65Pa.s, sodium iodide and N-methyl-2-pyrrolidone, then adding sodium hydroxide and methyl iodide, reacting, then carrying out ethanol precipitation, separating, and carrying out freeze drying, thereby obtaining N-trimethyl chitosan with the quaternization substitution degree of 10-20%; preparing N-trimethyl chitosan into solution, spraying into sodium polyphosphate, reacting, washing with water, adding into sodiumhydroxide solution, stirring, adding glutaraldehyde, and reacting, thereby obtaining the microsphere; and washing the microsphere with water, then sequentially eluting with organic solvents with thevolume fractions of 21-29%, 41-49%, 61-69% and 85-89%, and carrying out freeze drying. The modified chitosan prepared by adopting the method can reduce the dielectric loss of the vegetable oil.

The invention belongs to the field of feed additives and in particular relates to a veterinary chlortetracycline premix and a preparation method thereof. The premix is prepared from the following raw materials in parts by weight: 20 to 30 parts of chlortetracycline hydrochloride, 30 to 40 parts of N-trimethyl chitosan, 20 to 40 parts of starch, 2 to 6 parts of polyvinylpyrrolidone K30, 1 to 3 parts of a chitosan-EDTA (Ethylene Diamine Tetraacetic Acid) complex and 10 to 18 parts of water, and further comprises 4 to 8 parts of a sweetener; the sweetener is one of sucrose, lactose, sorbitol and mannitol. The premix is prepared into grains by adopting a wet-process granulation process and the prepared premix grains are uniform and have high granulation rate and stable quality. Furthermore, the premix can be used for effectively reducing complex compounds formed by the chlortetracycline hydrochloride and metal ions in gastrointestinal tracts; the chlortetracycline hydrochloride can be incompletely released in gastric juice and the bioavailability of the chlortetracycline hydrochloride is improved. The preparation process of the premix provided by the invention is simple and industrial application is easy to realize.

Gel nanoparticles for encapsulating and delivering a pharmaceutical compound to a patient. The nanoparticles are formed from N-trimethyl chitosan and polysialic acid, preferably in the presence of sodium tripolyphosphate. A ratio of polysialic acid to N-trimethyl chitosan of about 0.5 to 1 produces nanoparticles having diameter of about 100 nm (plus or minus 25 nm) and a zeta potential above 30 milivolts that can stability contain a pharmaceutical compound, such as methotrexate, for delivery to a patient.

The invention belongs to the field of macromolecular compounds and discloses a functional derivative, O-imidazate-N-trimethyl chitosan quaternary ammonium salt, prepared through chemical modification of chitosan, as well as a preparation method and application of the O-imidazate-N-trimethyl chitosan quaternary ammonium salt. The preparation method comprises the following steps: taking chitosan, formaldehyde and formic acid as starting materials, obtaining chitosan Schiff base through microwave reaction of the starting materials; then, carrying out reaction of the chitosan Schiff base and methyl iodide to obtain N,N,N-trimethyl chitosan; utilizing imidazole dicarboxylic acid and sulfoxide chloride to obtain imidazole acid acyl chloride; adding the imidazole acid acyl chloride into the N,N,N-trimethyl chitosan to obtain the O-imidazate-N trimethyl chitosan quaternary ammonium salt. The O-imidazate-N trimethyl chitosan quaternary ammonium salt is not only good in water-solubility, but also greatly higher in an antibiotic property, and can be applied to numerous application fields such as antibacterial materials, daily chemical products and industrial wastewater treatment.

The invention belongs to the field of the high polymer compound materials and discloses an O-thiazolidine methyl ester-N-trimethyl chitosan quaternary ammonium salt as well as a preparation method and application thereof. The preparation method of the O-thiazolidine methyl ester-N-trimethyl chitosan quaternary ammonium salt comprises the following steps: carrying out a microwave reaction on starting raw materials (including chitosan, formaldehyde and formic acid) to obtain a chitosan Schiff base and then reacting the chitosan Schiff base with methyl iodide to obtain N-trimethyl chitosan; preparing thiazolidine formyl chloride by using L-thiazolidine-4-formic acid and thionyl chloride, adding the thiazolidine formyl chloride to N-trimethyl chitosan to obtain the O-thiazolidine methyl ester-N-trimethyl chitosan quaternary ammonium salt. The O-thiazolidine methyl ester-N-trimethyl chitosan quaternary ammonium salt is greatly improved in term of antibacterial property in contrast with the chitosan, and thus can be applied to various fields such as antibacterial materials, chemical products of daily use and industrial wastewater treatment.

The invention relates to a preparation method and application of mannose-grafted trimethyl chitosan.The preparation method comprises: adding N,N,N-trimethyl chitosan into an organic solvent so that the organic solvent wells, adding mannopyran phenyl isothiocyanate for full reacting, and preparing N-mannose-N,N,N-trimethyl chitosan by ethanol washing, suction filtering and vacuum drying.The invention also provides application of the preparation method of the mannose-grafted trimethyl chitosan in the preparation of drug-carrying microspheres.The preparation method of a mannose-grafted trimethyl chitosan carrier has mild conditions, and a prepared mannose-grafted trimethyl chitosan drug-carrying microsphere preparation can target dendritic cells, overcoming mucosal barriers.

The invention belongs to the field of pharmaceutical preparations and particularly relates to a moxifloxacin hydrochloride external preparation and a preparation method thereof. The external preparation specifically refers to a cream and comprises a combination of a skin penetration enhancer comprising N-trimethyl chitosan, menthol and isopropyl myristate, the external preparation can be directly acted on an infection part, the gastric and intestinal irritation caused by oral administration is avoided, and the skin is not irritated either. The cream further greatly improves the skin penetration rate of a medicine, so that the local absorption is fast, and the treatment effect is remarkable. To further enhance the anti-inflammatory and anti-allergic effects, a non-steroidal anti-inflammatory drug, namely butyl flufenamate, is further added in the cream. The preparation process of the cream is simple and feasible, is high in reproducibility, and is suitable for industrial production.

The invention provides an immunopotentiator and a preparation method and an application thereof, wherein the immunopotentiator comprises at least one of chitosan, N,N,N-trimethyl chitosan quaternary ammonium salt and mannatide. The immunopotentiator can significantly improve immune response of immunized animals, increases the antibody level, thereby improving the immune effect of vaccines, shortening the immune window period and prolonging the protection period.

The invention belongs to the field of feed additives and in particular relates to a veterinary chlortetracycline premix and a preparation method thereof. The premix is prepared from the following raw materials in parts by weight: 20 to 30 parts of chlortetracycline hydrochloride, 30 to 40 parts of N-trimethyl chitosan, 20 to 40 parts of starch, 2 to 6 parts of polyvinylpyrrolidone K30, 1 to 3 parts of a chitosan-EDTA (Ethylene Diamine Tetraacetic Acid) complex and 10 to 18 parts of water, and further comprises 4 to 8 parts of a sweetener; the sweetener is one of sucrose, lactose, sorbitol and mannitol. The premix is prepared into grains by adopting a wet-process granulation process and the prepared premix grains are uniform and have high granulation rate and stable quality. Furthermore, the premix can be used for effectively reducing complex compounds formed by the chlortetracycline hydrochloride and metal ions in gastrointestinal tracts; the chlortetracycline hydrochloride can be incompletely released in gastric juice and the bioavailability of the chlortetracycline hydrochloride is improved. The preparation process of the premix provided by the invention is simple and industrial application is easy to realize.

The invention discloses a preparation method of a trinary composite stent of plasmid DNA / fibrin gel / polymer. The preparation method comprises the following steps of: geometrically and uniformly mixing an N-trimethyl chitosan solution and a plasmid DNA solution to prepare plasmid DNA composite particles; dissolving fibrinogen in physiological saline to prepare a fibrinogen solution; dissolving thrombin in a calcium chloride solution to prepare a thrombin calcium chloride solution; sterilizing a polymer porous stent with ethanol; simultaneously filling the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution with the same volumes in the polymer porous stent by utilizing negative pressure; and arranging the stent filled with the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution in a constant-temperature oven to fully gelatinize the fiber protein. The preparation method is simple and convenient, uses materials with wide resources and can simulate the composition and the structure of the cartilage of a human body. The composite stent of the invention has favorable mechanical property and biological activity, can accelerate the restoration of the cartilage and the osseous tissue and have strong application values of cartilage and bone restoration.