Method for extracting total RNA from sandalwood wood tissue

A wood and tissue technology, applied in the field of molecular biology, can solve problems such as difficulty in extracting total RNA from sandalwood wood tissue, and achieve the effects of strong promotion and application value, strong practicability, and good integrity.

Inactive Publication Date: 2016-05-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of high-quality method for extracting the total RNA o

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting total RNA from sandalwood wood tissue

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] The following plastic centrifuge tubes and pipetting heads used in the method of extracting total RNA from sandalwood wood tissue are soaked in chloroform for 30 minutes, then autoclaved at 121°C for 30 minutes, and dried for later use; tin foil for mortar and metal spoon Wrap it in paper and bake it in an oven at 180°C for 3 to 5 hours. DEPC water is treated by adding DEPC with a final concentration of 0.1% by mass to deionized double distilled water and sterilized by high temperature and high pressure.

[0021] The guanidine isothiocyanate extract, its formula is: 4mol / L guanidine isothiocyanate, 2mmol / LTris-HCl (pH8.0), 1% β-mercaptoethanol (v / v), and the solvent is water. The method is to mix the above ingredients uniformly and sterilize for later use.

[0022] SDS buffer, its formula is: 0.2% SDS (w / w), 100mmol / L NaCl, 50mmol / LTris-HCl (pH=8.0), 10mmol / LEDTA (pH=8.0), the solvent is water, its preparation method is to After the above ingredients are ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for extracting total RNA from sandalwood wood tissue. The method comprises the following steps: grinding taken sandalwood wood tissue into powder, adding the powder to a guanidinium isothiocyanate extract solution, and centrifuging after an ice bath; taking supernatant, adding an SDS buffer solution for mixing evenly, adding water-saturated phenol/chloroform/isoamyl alcohol for mixing evenly, and centrifuging the mixture; taking the supernatant, adding chloroform for mixing evenly and centrifuging the mixture; taking the supernatant, adding absolute ethyl alcohol and sodium acetate solution sediment RNA, and centrifuging the mixture for removing the supernatant; and taking the sediment and washing the sediment with ethanol to obtain the total RNA. The ratio of OD260 to OD280 of the extracted total RNA of the sandalwood wood tissue is 1.8 to 2.0; the ratio of OD260 to OD230 is 2.0 to 2.3; the concentration of the RNA water solution is 350.0-500.0ng/mu L; electrophoresis detection shows that the RNA strip is clear and good in integrity; and the brightness at 28S is 1.9-2.1 times that at 18S. The total RNA of the sandalwood wood tissue extracted by the method provides a good foundation for related researches of sandalwood molecular biology, and the method has relatively high popularization and application values.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a method for extracting total RNA from sandalwood wood tissue. Background technique [0002] Sandalwood (Santalum album L.) is a semi-parasitic evergreen tree of the genus Santalum of the family Santalaceae. It is a valuable and rare plant, mainly distributed in India, Indonesia, Australia and some islands in the Pacific Ocean. Humans have used it for thousands of years, and it is especially famous because its wood can be used for carving and refining sandalwood oil, known as "green gold". There is no native distribution of sandalwood in China, and its finished or semi-finished products have been imported from abroad in the past. In 1962, the South China Botanical Garden of the Chinese Academy of Sciences introduced sandalwood seeds from Indonesia to the mainland for the first time and succeeded in breeding. Later, it also successfully introduced the high-quality provenance of "...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 张新华牛美云程庆伟马国华
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap