Megakaryocyte progenitor cell cryopreservation solution and application thereof

A technology of megakaryotic progenitor cells and cryopreservation solution, which is applied in the field of bioengineering and can solve the problems of lack of cryopreservation research on megakaryotic progenitor cell types

Active Publication Date: 2016-06-01
ACADEMY OF MILITARY MEDICAL SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of research on cryopreservation for the specific cell type of megakaryotic progenitor cells

Method used

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  • Megakaryocyte progenitor cell cryopreservation solution and application thereof
  • Megakaryocyte progenitor cell cryopreservation solution and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The inventor adopts 7 kinds of cryopreservation solution containing only DMSO and culture medium, commercially available 07930 cryopreservation solution, plasma-free cryopreservation solution containing HSA, and plasma-containing cryopreservation solution of the present invention, respectively. The megakaryotic progenitor cells induced and expanded in vitro were cryopreserved, and the cryopreservation effect was compared to observe the effects of umbilical cord plasma and poloxamer on the cryopreservation of megakaryotic progenitor cells, as follows:

[0035] 1. Experimental method

[0036](1) Mononuclear cells from umbilical cord blood were isolated, megakaryotic progenitor cells were induced and expanded for 10 days in vitro, and expanded megakaryotic progenitor cells were obtained.

[0037] (2) The megakaryotic progenitor cells obtained on the 10th day of in vitro induction and expansion were cryopreserved in seven cryopreservation solutions, and the components of th...

Embodiment 2

[0045] The megakaryotic progenitor cells are cryopreserved according to the method of Example 1. The difference is that the frozen cells are megakaryotic progenitor cells induced in vitro for 14 days, and the components of part of the cryopreservation solution are different. For the specific components of the cryopreservation solution, see Table 2 .

[0046] Table 2 Effects of different cryopreservation solutions on cryopreservation of megakaryotic progenitor cells induced in vitro for 14 days (n=3)

[0047]

[0048] The results showed that plasma can effectively improve the survival rate of cryopreservation solution, and the number of recovered cells is large, and within a certain range, with the increase of plasma concentration, the survival rate of cells and the number of recovered cells also increased. Moreover, P188 and P407 can further increase the survival rate of cells and the number of recovered cells to a certain extent.

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Abstract

The invention discloses a megakaryocyte progenitor cell cryopreservation solution and application thereof. The megakaryocyte progenitor cell cryopreservation solution comprises DMSO (Dimethylsulfoxide) and blood plasma. The cryopreservation solution can be effectively used for cryopreserving megakaryocyte progenitor cells; after cell recovery, the survival rate and the recovery rate are high.

Description

technical field [0001] The present invention relates to the field of bioengineering, in particular, to a cryopreservation liquid for megakaryotic progenitor cells and applications thereof, more specifically, to a cryopreservation liquid for megakaryotic progenitor cells, a kit for cryopreserving megakaryotic progenitor cells, and a method for cryopreserving megakaryotic progenitor cells method. Background technique [0002] In recent years, the incidence of tumors has been on the rise. Thrombocytopenia is often found in the blood tests of patients undergoing radiotherapy and chemotherapy. Due to the shortage of blood sources and the need for repeated transfusions of platelets, it is necessary to find new sources of platelets. Umbilical cord blood hematopoietic stem / progenitor cells can differentiate into megakaryotic progenitor cells, and further mature to produce platelets. Therefore, it is expected to increase the number of platelets in the blood by infusing megakaryotic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0226
Inventor 裴雪涛陈琳刘大庆岳文李雪潘志婷黄安平陈志清吕洋
Owner ACADEMY OF MILITARY MEDICAL SCI
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