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Application of protocatechuic acid to improving immune function of livestock

A protocatechuic acid, immune function technology, applied in medical preparations containing active ingredients, organic active ingredients, allergic diseases, etc.

Inactive Publication Date: 2016-06-01
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, protocatechuic acid has less reports on improving cell function (especially T lymphocytes) and antiviral activity

Method used

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  • Application of protocatechuic acid to improving immune function of livestock
  • Application of protocatechuic acid to improving immune function of livestock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Effect of protocatechuic acid on poultry immune function

[0021] 40 and 20 mg / kg body weight doses of PCA were fed to 21-day-old SPF chickens for 7 consecutive days, the peripheral blood and thymus were aseptically collected, lymphocytes were separated, and the ratio of CD4+T / CD8+T was detected by flow cytometry ;Calculation of organ index of thymus, spleen and bursa; Thymus and bursa were immediately frozen in liquid nitrogen for transcriptome sequencing; Simultaneous detection of TGF, IL-12, IL-10, IL-8, IL in thymus and spleen -6, IL-4, IL-2 mRNA levels of TNF, MHC-1, MHC-2, IFN-α, IFN-γ, IL-15 and MDA5.

[0022] 1. Organ index

[0023] Table 1 Effects of protocatechuic acid treatment on body weight and organ indices

[0024]

[0025] Table 1 has shown the influence of feeding medicine on body weight and organ index of SPF chickens after 7 days, the results show that the weight gain rate of protocatechuic acid 40mg / kg group, 20mg / kg group, blank contr...

Embodiment 2

[0036] Example 2 Effect of Protocatechuic Acid on Livestock Immune Function

[0037] 20 weaned piglets were randomly selected and divided into PCA group and healthy control group. PCA was continuously fed with 20 mg / kg body weight mix for 7 days. Peripheral anticoagulant blood was collected aseptically and tested by flow cytometry.

[0038] The results showed that after feeding 20mg / kg protocatechuic acid for 7 days, the ratio of CD4+ / CD8+ in peripheral blood changed a lot, and the ratio of CD4 / CD8 in the protocatechuic acid group reached 1.9±0.2, which was significantly higher than that in the healthy control group 1.2±0.2 (P<0.01).

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Abstract

The invention discloses new application of protocatechuic acid in the aspect of immune function promotion of livestock, especially in the aspects of peripheral blood CD4+ / CD8+T cell dynamic balance maintenance of livestock, cytokine secreting adjustment and the like. Influences of protocatechuic acid on the immune function, especially on T cell differentiation, of livestock are evaluated, 20 mg of protocatechuic acid is applied to each kilogram of body weight, protocatechuic acid is continuously taken orally for seven days, and results show that protocatechuic acid can effectively increase the peripheral blood CD4+ / CD8+T ratio, promote cytokine secreting in thymuses and the spleen and selectively adjust related genes of the immune system. The invention further provides a livestock immune promoter containing an effective dose of protocatechuic acid. Medical auxiliary materials can be added and prepared into various preparations, such as powder, tablets, granules and oral liquid, according to a conventional preparation method in the field. The effective dose is 5-40 mg for each kilogram of body weight, and the preparation is taken in a mixing mode or with water.

Description

technical field [0001] The invention relates to biopharmaceutical technology, in particular to the application of protocatechuic acid in improving the immune function of livestock and poultry. Background technique [0002] Protocatechuic acid (PCA), chemical name is 3,4-dihydroxybenzoic acid, its basic information is CAS: [99-50-3], molecular formula: (HO) 2 C 6 h 3 COOH, molecular weight: 154.12. It is an important phenolic acid compound, which has multiple pharmacological effects, can improve the efficacy of antiviral drugs, and has anti-inflammatory and tissue damage repair effects. [0003] However, protocatechuic acid has less reports on improving cell function (especially T lymphocytes) and antiviral activity. In improving cell function, Miroslava reported that grape seed extract rich in phenolic acids (including protocatechuic acid) has a very good effect on preventing oxidative lymphocyte damage caused by ROS, while D and others showed that grape seed extract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61P37/04
CPCA61K31/192
Inventor 欧长波王秋霞余燕张艳红姜金庆姚四新崔艳红马金友胡建和刘兴友
Owner HENAN INST OF SCI & TECH
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