Extraction method for entity small molecule peptide of pearls

A technology of small molecule peptide and extraction method, which is applied in the field of pearl entity small molecule peptide extraction, can solve the problem that small molecule peptide extraction is rarely mentioned, and achieves the effects of improving human immunity, rapid absorption and good health care effect.

Inactive Publication Date: 2016-06-01
蔡庭守
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the development of human society, people are paying more and more attention to health, and the value of small molecular peptides has also been valued by people, but there is little mention of the extraction of small molecular peptides in pearls

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting pearl entity small molecule peptides, comprising the steps of:

[0023] Step 1. Use a low-temperature vacuum drying oven to dry the pearls at 50°C for 15 hours, and then crush them into powder with a pulverizer to obtain pearl powder with a particle size of 80 mesh;

[0024] Step 2, mix the pearl powder and water according to the mass ratio of 1:10, stir and heat to 80°C, keep warm for 15min, then cool down to 50°C, add alkaline protease with enzyme activity of 500,000 u / g, the The amount of alkaline protease added is 1% of the mass of the pearl powder; then the pH is adjusted to 9 with a NaOH solution with a mass fraction of 10%, and the temperature is kept at 50° C. for 2 hours to obtain an alkaline solution;

[0025] Step 3, adding enzyme activity to the alkaline solution is a neutral protease of 150,000 u / g, and the amount of the neutral protease added is 1% of the pearl powder mass; then adopting a mass fraction of 10% HCl solution Adjust t...

Embodiment 2

[0030] A method for extracting pearl entity small molecule peptides, comprising the steps of:

[0031] Step 1. Use a low-temperature vacuum drying oven to dry the pearls at 60°C for 20 hours, and then crush them into powder with a pulverizer to obtain pearl powder with a particle size of 120 mesh;

[0032] Step 2, mix the pearl powder and water according to the mass ratio of 1:15, stir and heat to 100°C, keep warm for 15min, then cool down to 50°C, add alkaline protease with an enzyme activity of 500,000 u / g, the The amount of alkaline protease added is 1% of the mass of the pearl powder; then the pH is adjusted to 10 with a NaOH solution with a mass fraction of 10%, and the temperature is kept at 50° C. for 3 hours to obtain an alkaline solution;

[0033] Step 3, adding enzyme activity to the alkaline solution is a neutral protease of 150,000 u / g, and the amount of the neutral protease added is 1% of the pearl powder mass; then adopting a mass fraction of 10% HCl solution Ad...

Embodiment 3

[0038] A method for extracting pearl entity small molecule peptides, comprising the steps of:

[0039] Step 1. Use a low-temperature vacuum drying oven to dry the pearls at 55°C for 18 hours, and then pulverize them into powder with a pulverizer to obtain pearl powder with a particle size of 100 mesh;

[0040] Step 2, mix the pearl powder and water according to the mass ratio of 1:12, stir and heat to 90°C, keep warm for 15min, then cool down to 50°C, add alkaline protease with an enzyme activity of 500,000 u / g, the The amount of alkaline protease added is 1% of the mass of the pearl powder; then the pH is adjusted to 9.7 with a 10% NaOH solution, and the temperature is kept at 50°C for 2.5 hours to obtain an alkaline solution;

[0041] Step 3, adding enzyme activity to the alkaline solution is a neutral protease of 150,000 u / g, and the amount of the neutral protease added is 1% of the pearl powder mass; then adopting a mass fraction of 10% HCl solution Adjust the pH to 7.3, ...

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Abstract

The invention relates to an extraction method, in particular to an extraction method for entitysmall molecule peptide of pearls. The extraction method comprises the following steps: drying the pearls; smashing the dried pearls to obtain pearl powder; mixing the pearl powder with water, carrying out stirring, heating and cooling, adding basic protease, and adjusting pH to obtain an alkaline solution; adding neutral protease into the alkaline solution, and adjusting pH to obtain a neutral solution; adding pepsase into the neutral solution, and adjusting pH to obtain an acid solution; heating the acid solution, and carrying out filtering to obtain filtrate; adding activated carbon into the filtrate for decolorization, and carrying out filtering, concentrating and drying to obtain entity small molecule peptide powder. The extraction method for the entity small molecule peptide adopts a compound enzyme enzymolysis technology, and has the advantage that the molecular weight of the extracted small molecule peptide is mainly 180 to 480 dalton; the extracted small molecule peptide is mainly dipeptide, tripeptide, tetrapeptide and the like, and belongs to small molecular structures, thereby being high in absorbing and utilizing possibilities.

Description

technical field [0001] The invention relates to an extraction method, in particular to an extraction method of pearl entity small molecule peptide. Background technique [0002] Pearl is an ancient organic gemstone, mainly produced in the molluscs of pearl shellfish and pearl mollusks; and the mineral beads containing calcium carbonate produced by endocrine action are composed of a large number of tiny aragonite crystals . The main component of pearls is calcium carbonate. In addition, pearls also contain more than 20 kinds of trace elements, vitamin B and a variety of proteins. After protein hydrolysis, 18 kinds of amino acids can be obtained, 7 of which are essential amino acids for human body. [0003] Generally, 2-6 amino acids can form oligopeptides, and the molecular weight of oligopeptides is generally below 1000 Daltons, while small molecule peptides are ultra-low molecular weight oligopeptides, highly active peptides composed of only 2-4 amino acids, and the molecu...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 蔡庭守
Owner 蔡庭守
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