Method for preparing three-dimensional penetrating vessel-like structure network scaffold by drum collection

A technology of vascular structure and structural network, applied in the direction of prosthesis, tissue regeneration, additive processing, etc., can solve the problem of staying in two-dimensional sheets, achieve high efficiency, simple process, and promote the effect of integration

Active Publication Date: 2019-01-11
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter which method is used, the construction of the three-dimensional network has not been completely solved. At present, it mainly stays in the construction of the two-dimensional sheet-through network.

Method used

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  • Method for preparing three-dimensional penetrating vessel-like structure network scaffold by drum collection
  • Method for preparing three-dimensional penetrating vessel-like structure network scaffold by drum collection
  • Method for preparing three-dimensional penetrating vessel-like structure network scaffold by drum collection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] see Figure 1 ~ Figure 4 , the drum-type collection method for preparing a three-dimensional through-structure network scaffold: such as figure 1 , the three-dimensional solid network preparation system provides the required material driving force by gears (1-2) and rollers (1-3); uses heating modules (1-4) and extrusion nozzles (1-5) as feeding modules; adopts Cylindrical drums as collection devices, such as figure 2 with image 3 ; Utilize the water-soluble characteristics of silk PVA, sacrifice it after it is formed; the preparation process is as follows:

[0030] 1) Preparation of materials and stock solution: non-toxic water-soluble silk is selected as the material of the three-dimensional solid network; the hydrogel is dissolved in a hot water bath;

[0031]2) Material extrusion: For material extrusion, heat the heating nozzle to the set temperature, dissolve the filament pushed to this module, and extrude it from the extrusion nozzle;

[0032] 3) Preparation...

Embodiment 2

[0036] This embodiment is basically the same as Embodiment 1, and the special features are as follows: the step 1) preparation of materials and stock solution: the three-dimensional solid network material is selected from non-toxic water-soluble silk polyvinyl alcohol PVA; The method of hot water bath dissolves it. Due to this feature, if the stent is transplanted into the body, the stent will dissolve. Therefore, in order to improve the stability of this stent, glutamine is added to the hydrogel to improve its gel properties. Transaminase TG improves its gelation degree and avoids dissolution after molding; its preparation method is to first prepare a gelatin solution with a mass ratio of 10±0.5%, dissolve it in a hot water bath at 60±2°C, and wait until it becomes When it is transparent, add transglutaminase powder and stir well before use. The ratio of gelatin and transglutaminase in this hydrogel is 10:1~1.1.

[0037] Step 2) material extrusion: material extrusion, heating...

Embodiment 3

[0040] Example 3: Preparation of Bone Tissue Structure

[0041] In this case, cell manipulation is involved, which needs to be carried out in a sterile environment. Therefore, the experimental equipment and materials should be sterilized before the experiment. The experiment is carried out in an ultra-clean bench. The specific steps are as follows:

[0042] 1) Preparation of materials and stock solution: The three-dimensional solid network material is made of non-toxic water-soluble silk polyvinyl alcohol PVA, which is sterilized by ultraviolet light for at least 30 minutes before use; the hydrogel is made of gelatin, which is dissolved in a hot water bath , due to this feature, if the stent is transplanted into the body, the stent will dissolve. Therefore, in order to improve the stability of this stent, transglutaminase TG, which improves its gel properties, is added to the hydrogel to improve its gelation. to avoid dissolution after molding; the preparation method is to fir...

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Abstract

The invention discloses a method for preparing a three-dimensional interconnected blood vessel-liked structure network scaffold by roller type collection. The method is based on hot-melt extrusion of water-soluble materials, the roller type collection mode is used for forming a prefabricated three-dimensional entity network, a hydrogel material is used for embedding, after moulding, the material is placed in water, the three-dimensional entity network is removed, and the three-dimensional interconnected blood vessel-liked structure network is formed in the hydrogel. A three-dimensional idea for traditional connection between layers is broken through, the characteristics of the cylindrical roller is used, the cylindrical surface is used for receiving the water-soluble material, and the entity network with the three-dimensional structure which is formed after extrusion is constructed.

Description

Technical field: [0001] The invention relates to a construction method for preparing a three-dimensional penetrating blood vessel-like structure network support through drum-type collection, and belongs to the field of tissue engineering support preparation. Background technique: [0002] Bone defect has always been one of the major health problems faced by human beings. With the development of tissue engineering technology, different types of bone tissue scaffolds for bone tissue repair are emerging. However, the structure of bone tissue is complex, and there are still great problems in the construction of functional bone tissue scaffolds. The most important issue is to solve the problem of ischemic necrosis of tissue engineering scaffolds. [0003] In recent years, biomanufacturing with 3D printing technology as the core provides a technical basis for the rapid and direct construction of the macroscopic geometry of living structures. Bone tissue vascularization is of grea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/38A61L27/22B33Y30/00B33Y10/00
CPCA61L27/222A61L27/227A61L27/38A61L27/52A61L2430/02B33Y10/00B33Y30/00C08L89/00
Inventor 胡庆夕李帅刘媛媛孙元劭李瑜刘嫦娥
Owner SHANGHAI UNIV
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