Probiotics composition with fat reducing function and preparation method and application thereof
A technology of probiotic bacteria and composition, applied in the field of biomedicine, can solve the problems of rebound, large body damage, poor effect, etc., and achieve the effects of simple preparation process, high safety, and avoiding drug loss
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Embodiment 1
[0037] (1) Prepare probiotic dry powder by selecting materials:
[0038] 1) Bifidobacteria, Lactobacillus plantarum, and Lactobacillus acidophilus were respectively activated in the culture medium, inoculated in the fermentation medium, and cultured with shaking, so that the concentration of the bacteria reached the logarithmic growth phase of 0.5 to 1.0×10 9 cfu / ml;
[0039] Preparation of Bifidobacterium seed solution: Take out the strain preservation tube, use Bifidobacterium solid medium to scratch the plate for recovery, and culture anaerobically at 37 degrees for 72 hours. Pick a single colony under the plate and inoculate it in 50 ml of bifidobacterium medium, and culture it anaerobically at 37 degrees in an incubator for 48 hours. The seeds are inoculated into a 10L large triangular flask with an inoculum volume of 5% (the liquid volume is 9.5L), cultured anaerobically at 37 degrees for 20-28 hours, and the concentration of the bacteria liquid is detected. The bacteri...
Embodiment 2
[0052] The specific steps are the same as in Example 1, the difference being: ingredients in step (3): Weigh and sieve raw materials according to 50% of probiotic dry powder, 10% of honeysuckle extract powder, 5% of Artemisia annua extract powder, Astragalus membranaceus 10% of the extract powder, 15% of the pueraria root extract powder, and 10% of the Huangling extract powder are used as ingredients, and the above contents are all percentages by weight.
Embodiment 3
[0054] The specific steps are the same as in Example 1, the difference being: ingredients in step (3): Weigh and sieve the raw materials according to 50% of probiotic dry powder, 5% of honeysuckle extract powder, 5% of Artemisia annua extract powder, Astragalus membranaceus 15% of the extract powder, 10% of the pueraria root extract powder, and 15% of the Huangling extract powder are used as ingredients, and the above contents are all percentages by weight.
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