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Zein and glycopolypeptide graft and its preparation method and application

A technology of zein and grafts, which is applied in the field of chemical modification of natural protein and its medical application, can solve the problems of unseen research work, and achieve good cell growth, good biocompatibility and degradability , Good adhesion and growth effect

Active Publication Date: 2018-07-03
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, glycopolypeptide biomimetic polymers have been used to prepare bioactive polymer vesicles and polymer micelles, but there is no research work on using it to modify zein to construct tissue engineering specific cell scaffold material

Method used

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  • Zein and glycopolypeptide graft and its preparation method and application
  • Zein and glycopolypeptide graft and its preparation method and application
  • Zein and glycopolypeptide graft and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of zein and glycopolypeptide graft (ZG-1) and its electrospun membrane

[0045] (1) Preparation of galactose pentaacetate

[0046] D-galactose (5.00 g) was dissolved in 15 mL of pyridine, and acetic anhydride (27 mL) was added at 0° C. under nitrogen protection. After reacting for 24 h, it was slowly poured into ice water (100 mL), and extracted three times with ethyl acetate. The obtained organic phase was washed with saturated NaHCO 3 The solution was washed until no gas was generated, and then washed with water and concentrated brine three times in sequence. The organic phase was dried with anhydrous magnesium sulfate and then rotary evaporated to obtain an oily product with a yield of 97%.

[0047] (2) Preparation of 3-ynbutyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside

[0048] Galactose pentaacetate (10.00g) and 3-butyn-1-ol (2.3mL) were dissolved in 50mL of dry dichloromethane, and Et was added dropwise at 0°C under nitrogen protection ...

Embodiment 2

[0066] Example 2 Preparation of zein and glycopolypeptide graft (ZG-2) and its electrospun membrane

[0067] (1) Preparation of galactose pentaacetate

[0068] D-galactose (5.00 g) was dissolved in 15 mL of pyridine, and acetic anhydride (27 mL) was added at 0° C. under nitrogen protection. After reacting for 24 h, it was slowly poured into ice water (100 mL), and extracted three times with ethyl acetate. The obtained organic phase was washed with saturated NaHCO 3 The solution was washed until no gas was generated, and then washed with water and concentrated brine three times in sequence. The organic phase was dried with anhydrous magnesium sulfate and then rotary evaporated to obtain an oily product with a yield of 97%.

[0069](2) Preparation of 3-ynbutyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside

[0070] Galactose pentaacetate (10.00g) and 3-butyn-1-ol (2.3mL) were dissolved in 50mL of dry dichloromethane, and Et was added dropwise at 0°C under nitrogen protection ...

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Abstract

The invention discloses a zein and glycopolypeptide grafted copolymer and a preparation method and application thereof. The preparation method comprises the steps that firstly, inherent amidogens of zein macromolecules are utilized to trigger an N-carboxyanhydride monomer containing azide functional groups for ring opening polymerization, azide-containing side-group polypeptide modified zein is obtained, and galactose is grafted to a polypeptide chain segment side group so as to obtain the zein and glycopolypeptide grafted copolymer. The zein and glycopolypeptide grafted copolymer prepared by means of the preparation method can be used for preparing a corresponding electrospun fibrous membrane serving as a liver cell scaffold material by adopting an electrospinning technique. The main raw material used for the zein and glycopolypeptide grafted copolymer belongs to a natural vegetable protein, a grafted glycopolypeptide chain segment is of a chemical structure similar to a glycoprotein in a living body, and therefore the prepared zein and glycopolypeptide grafted copolymer and the electrospun membrane scaffold material thereof have very good biocompatibility and bionic characteristics.

Description

technical field [0001] The invention belongs to the field of chemical modification of natural protein and its medical application, and in particular relates to a zein-glycopolypeptide graft, a preparation method thereof and its application in the construction of liver cell scaffold materials. Background technique [0002] Zein, which is derived from major crops, is a kind of natural polymer with large quantity, wide range, non-toxicity and easy biodegradation. In recent years, it has been used as biomedical materials, especially the modification and high-value utilization of tissue engineering scaffold materials. , is gradually attracting great attention from scholars at home and abroad. Compared with synthetic polymer raw materials commonly used in the construction of tissue engineering scaffolds, such as polylactic acid, polycaprolactone, polycarbonate, polyglycolic acid, etc., this kind of natural polymer is not only rich in raw materials, renewable and good biocompatibil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G81/00C12N5/071
CPCC08G81/00C12N5/067C12N2533/50
Inventor 张黎明缪迎羚
Owner SUN YAT SEN UNIV
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