Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof

Abstract

The invention relates to a strain of bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and a low cost production method and application method of the inoculant of bacillus subtilis, and belongs to the technical fields of agricultural microbial inoculant and energy saving and emission deduction. The adopted strain is a SZX11 strain of bacillus subtilis. The SZX strain is separated and screened from pine needles, and is preserved in China General Microbiological Culture Collection Center (CGMCC). The preservation number is CGMCC No.8188. Sweet potato starch wastewater is taken as the liquid fermentation medium to massively produce bacillus subtilis and prepare agricultural microbial inoculants; the technology has the advantages of low energy consumption, low production cost, and simple technology, moreover, the sweet potato starch wastewater is utilized, and the pollutant discharge is reduced. The produced inoculant can be added into the irrigation water of irrigation system, is capable of reliving the obstruction of irrigation devices, can also improve the quality of agricultural products, and has a wide application prospect.

Description

Technical field: [0001] The invention relates to a strain of Bacillus subtilis (Bacillus subtilis) SZX11 capable of efficiently degrading quorum sensing signal molecules, and a low-cost production method thereof, belonging to the technical fields of agricultural microbial bacterial agents and energy saving and emission reduction. Background technique: [0002] A typical behavior of quorum sensing is biofilm formation. When microbial individuals reach a certain density, gene expression of quorum-sensing signaling molecules produces mucus polysaccharides, leading to the formation of structural biofilms. Earlier research literature on bacterial quorum sensing focused on the study of pathogenic bacteria. When bacteria reach a high enough population density, they can change the expression of genes required for infection to induce a large number of cells to cooperate for host infection. They can also evade the host. immune response to successfully establish infection (Dangl, et a...

AI Technical Summary

Problems solved by technology

However, the current processing technology produces about 10 tons of high-concentration organic wastewater for every 1 ton of sweet potato starch produced. Among them, the sweet potato cell fluid after starch separation has the highest organic content, and the COD can reach more than 20,000 mg / L, mainly containing soluble starch. , protein, pectin, organic acid and a small amount of oil, if not handled properly, it is easy to be spoiled and fermented, making the water black and smelly

However, the general sewage treatment process is used to treat such high-concentration wastewater to meet the standard discharge, and the energy consumption is very large.