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Colloidal-gold-based quantitative mycotoxin detection device and preparation method

A technology for quantitative detection of mycotoxins, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of reducing the actual guidance effect of production, and achieve the effects of fast detection time, wide application range and less error

Inactive Publication Date: 2016-07-20
成都盛泰尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is fast and accurate, but requires expensive instruments and equipment. It is limited to professional testing institutions for scientific research, investigation, analysis, and monitoring, and has not been widely used in enterprises and grassroots. Moreover, the hysteresis effect of the results greatly reduces the impact on actual production. Guidance effect

Method used

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  • Colloidal-gold-based quantitative mycotoxin detection device and preparation method
  • Colloidal-gold-based quantitative mycotoxin detection device and preparation method
  • Colloidal-gold-based quantitative mycotoxin detection device and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] The aflatoxin B1 colloidal gold quantitative detection device includes a colloidal gold quantitative detection box and a hand-held card reader. The detection device can give accurate quantitative detection results for the toxin product to be detected.

[0067] The quantitative detection result is based on the ratio of the color intensity of the detection line T line measured by the hand-held card reader to the color intensity of the C line control and the content of the mycotoxin in the sample to be tested is related to a quantitative function relationship, the function is Curve or logarithmic function relationship, set as the internal standard curve of the instrument.

[0068] Aflatoxin B1 colloidal gold quantitative test kit, including cartridge and reagent strip, the reagent strip is composed of substrate, sample pad, colloidal gold pad, reaction membrane and water-absorbing pad, colloidal gold-labeled aflatoxin B1 is adsorbed on the colloidal gold pad Monoclonal ant...

Embodiment 2

[0109] The zearalenone colloidal gold quantitative detection device includes a colloidal gold quantitative detection box and a hand-held card reader. The detection device can give accurate quantitative detection results for the toxin product to be detected.

[0110] The quantitative detection result is based on the ratio of the color intensity of the detection line T line measured by the hand-held card reader to the color intensity of the C line control and the content of the mycotoxin in the sample to be tested is related to a quantitative function relationship, the function is Curve or logarithmic function relationship, set as the internal standard curve of the instrument.

[0111] Colloidal gold quantitative detection kit for zearalenone, including a cartridge and a reagent strip. The reagent strip is composed of a substrate, a sample pad, a colloidal gold pad, a reaction membrane and a water-absorbing pad. Colloidal gold-labeled zearalen is adsorbed on the colloidal gold pa...

Embodiment 3

[0148] The vomitoxin quantitative colloidal gold detection device includes a colloidal gold quantitative detection box and a hand-held card reader. The detection device can give accurate quantitative detection results for the toxin product to be detected.

[0149] The quantitative detection result is based on the ratio of the color intensity of the detection line T line measured by the hand-held card reader to the color intensity of the C line control and the content of the mycotoxin in the sample to be tested is related to a quantitative function relationship, the function is Curve or logarithmic function relationship, set as the internal standard curve of the instrument.

[0150] DON quantitative colloidal gold detection kit, including cartridges and reagent strips, the reagent strip is composed of a substrate, a sample pad, a colloidal gold pad, a reaction membrane and a water-absorbing pad, colloidal gold-labeled deoxynivalenol monoclonal antibody and cattle are adsorbed on...

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Abstract

The invention belongs to the field of biological detection, and particularly relates to a colloidal-gold-based quantitative mycotoxin detection device and a preparation method. The detection device comprises a colloidal-gold-based quantitative detection kit and a handheld card reader, and by the detection device, an accurate quantitative detection result is provided for a toxin product to be detected. The detection kit comprises a cartridge and a reagent strip, wherein the reagent strip consists of a substrate, a sample pad, a colloidal gold pad, a reaction film and an absorbent pad; a colloidal gold labeled mycotoxin and bovine serum albumin are adsorbed to the colloidal gold pad; the reaction film is coated with a detection antigen and a quality control antibody, the detection antigen is displayed as a detection line T, and is a mycotoxin antigen, and the quality control antibody is displayed as a quality control line C, and is a goat anti-mouse IgG antibody. The detection device can be operated conveniently and rapidly, is applicable to various kinds of field detection, and is matched with a colloidal-gold-based quantitative analyzer and a specific curve establishment method to quantitatively detect the content of a sample by virtue of colloidal gold and integrate the dual advantages of the colloidal gold and ELISA to make the two technologies complementary.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a mycotoxin quantitative detection device and a preparation method. Background technique [0002] Mold is a multicellular microorganism that widely exists in nature and belongs to fungi in microbiology. It reproduces in the form of spores. Mycotoxins are poisonous and harmful substances produced by molds or fungi. Mycotoxins can be found in soil and on plants, including grain, forage and silage. Environmental conditions of high temperature and high humidity are generally conducive to the growth of molds and the production of mycotoxins in feed. [0003] More than 300 mycotoxins have been isolated and identified so far. Generally speaking, mycotoxins are mainly produced by four kinds of molds: Aspergillus (mainly secrete aflatoxin, ochratoxin, etc.), Penicillium (mainly secrete citrinin, etc.), ergot (mainly secrete Ergot toxin), Fusarium sp. (mainly secrete ze...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
CPCG01N33/577G01N33/558
Inventor 蔡文胜刘文副李丹胡俊峰徐江波马路春
Owner 成都盛泰尔生物科技有限公司
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