A kind of Achromobacter cz207 bacterial strain that resists heavy metal and promotes plant growth and application thereof
A technology of promoting plant growth, CZ207, which is applied in the field of plant growth promotion and bioremediation of heavy metal polluted environment, which can solve the problems of less remediation, slow growth speed and single absorption of heavy metals
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[0009] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.
[0010] Isolation and identification of strains
[0011] In this example, a plant rhizosphere microorganism was isolated from the plant rhizosphere soil grown in a certain iron ore factory in Enshi Autonomous Prefecture, and it was named CZ207. The morphology, physiological and biochemical characteristics of the strain were as follows: observed on LB solid medium The colony was light yellow, neat, moist, and raised in the center; the bacterial strain was Gram-negative, rod-shaped, and without spores under the microscope.
[0012] Inoculate the CZ207 strain into 5ml LB culture solution (peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, H 2 (01000mL), 37 ℃, 200rpm shaker culture 24 hours, 10000rpm centrifugation 1min, collect thalline in 1.5ml centrifuge tube, adopt CTAB method to extract ...
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