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A kind of Achromobacter cz207 bacterial strain that resists heavy metal and promotes plant growth and application thereof

A technology of promoting plant growth, CZ207, which is applied in the field of plant growth promotion and bioremediation of heavy metal polluted environment, which can solve the problems of less remediation, slow growth speed and single absorption of heavy metals

Inactive Publication Date: 2019-04-16
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with traditional heavy metal remediation such as physical chemistry, phytoremediation has the advantages of simple operation, good treatment effect, no secondary pollution, low remediation cost, and large-scale promotion and application. It has good social and ecological comprehensive benefits, but phytoremediation The method also has bottleneck problems such as slow growth rate, small amount of restoration, easy to be limited by natural and artificial factors, and relatively simple absorption of heavy metals.

Method used

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  • A kind of Achromobacter cz207 bacterial strain that resists heavy metal and promotes plant growth and application thereof
  • A kind of Achromobacter cz207 bacterial strain that resists heavy metal and promotes plant growth and application thereof

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Embodiment Construction

[0009] The present invention will be described in detail below in conjunction with specific examples, but the protection scope of the present invention is not limited to the following examples.

[0010] Isolation and identification of strains

[0011] In this example, a plant rhizosphere microorganism was isolated from the plant rhizosphere soil grown in a certain iron ore factory in Enshi Autonomous Prefecture, and it was named CZ207. The morphology, physiological and biochemical characteristics of the strain were as follows: observed on LB solid medium The colony was light yellow, neat, moist, and raised in the center; the bacterial strain was Gram-negative, rod-shaped, and without spores under the microscope.

[0012] Inoculate the CZ207 strain into 5ml LB culture solution (peptone 10.0g, yeast extract 5.0g, NaCl 10.0g, H 2 (01000mL), 37 ℃, 200rpm shaker culture 24 hours, 10000rpm centrifugation 1min, collect thalline in 1.5ml centrifuge tube, adopt CTAB method to extract ...

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Abstract

The invention provides a plant growth-promoting Achromobacter sp CZ207 strain resistant to heavy metals such as cadmium, copper, lead, nickel, manganese, etc., which was preserved in the China Center for Type Culture Collection on March 7, 2016. The address is: Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the deposit number is CCTCC NO: M2016091. The 16S rDNA sequence of the strain is shown in SEQ ID NO.1. The bacterial colonies observed on LB solid medium were light yellow, neat, moist, and raised in the center; the strains observed under the microscope were Gram-negative, rod-shaped, and without spores. The above-mentioned CZ207 bacterial strain provided by the present invention can tolerate various heavy metals, and can secrete plant growth hormone, dissolve inorganic phosphorus, fix nitrogen and other nutrient elements, and have the effect of promoting plant growth.

Description

technical field [0001] The invention relates to a new strain of Achromobacter, which can tolerate multiple heavy metals such as chromium and cadmium, and particularly relates to the plant growth-promoting effect of the strain and the bioremediation of heavy metal-polluted environments. Background technique [0002] With the development of the economy, the demand for heavy metals in all walks of life is increasing day by day. Wastes from industrial metallurgy and electroplating, urban domestic sewage containing heavy metals, and agricultural fertilizers and pesticides have become the main channels for heavy metals to enter the environment. Heavy metal pollution not only reduces soil fertility, inhibits the growth and reproduction of soil microorganisms, affects the yield and quality of crops, but also affects the health of animals and humans through the food chain. Traditional heavy metal repair mainly adopts physical and chemical methods, such as precipitation method, ion e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P21/00B09C1/10C12R1/025
CPCA01N63/10B09C1/10C12N1/205C12R2001/025
Inventor 程国军田梦洋曾小波杜冬云何冬兰李晓华殷杰彭杨
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES