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Two pairs of Ustilago esculenta for successful invasion of zizania aquatica plant and breeding of zizania aquatica and artificial inoculation method thereof

A technology of black powder fungus and water jasmine, which is applied in the field of artificial inoculation of water jasmine, can solve the problems of unsuccessful artificial inoculation, etc., and achieve the effect of simple operation.

Active Publication Date: 2016-08-10
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far there have been no reports of successful artificial inoculation

Method used

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  • Two pairs of Ustilago esculenta for successful invasion of zizania aquatica plant and breeding of zizania aquatica and artificial inoculation method thereof
  • Two pairs of Ustilago esculenta for successful invasion of zizania aquatica plant and breeding of zizania aquatica and artificial inoculation method thereof
  • Two pairs of Ustilago esculenta for successful invasion of zizania aquatica plant and breeding of zizania aquatica and artificial inoculation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Screening of Ustilago esculenta haploid strain UET1 and Ustilago esculenta haploid strain UET2, such as figure 1 shown;

[0022] 1. Collect teliospores: from Longjiao No. 2 gray bamboo ( figure 1 A) Pick teliospores directly.

[0023] 2. Teliospore suspension: pile the picked teliospores into 5 mL sterile water, filter through 4 layers of sterile gauze to obtain a relatively pure teliospore suspension, and finally dilute the teliospore suspension with sterile water Dilute to a final concentration of 10 3 spores / mL. Teliospores form as figure 1 Shown in B.

[0024] 3. Teliospore culture: Take 100 µL of teliospore suspension and spread it on the basidiospore isolation medium, and culture it at 28°C for about 60 hours, finely dispersed single colonies can be seen with the naked eye ( figure 1 C) as standard. Basidiospore isolation medium (1 L) formula is: K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5 g, FeSO 4 ·7H 2 O 0.01g, KCl 0.5 g, glucose 18 g, (NH 4 ) 2...

Embodiment 2

[0043] Embodiment 2: the artificial inoculation method that makes Zizania zizania plant pregnant Zizania

[0044] 1) Shake the haploid strain of Ustilago smut in YEPS liquid medium (2% peptone, 2% sucrose, 1% yeast powder and the rest of water) at 25-30°C to OD 600 0.8-1.0, the cells were collected by centrifugation, and diluted with 0.5×YEPS liquid medium to a final concentration of OD 600 For the 2.0-3.0 bacterium liquid, the bacterium liquid of the haploid strain of the smut fungus is mixed for inoculation;

[0045] 2) Cultivate the tube-shaped roots of wild asparagus with more than 3 complete internodes, and cultivate them in the greenhouse for 15-20 days. The tubular root of the above small seedling stage plant is the inoculation object, and the base of the seedling is pricked;

[0046] 3) Inject the mixed bacterial liquid obtained in step 1) into the tubular root with a syringe until the bacterial liquid overflows;

[0047] 4) Trim the treated inoculated seedlings to ...

Embodiment 3

[0050] Example 3: Screening of strains of Ustilago smilax that can successfully infect Zizania zizania plants and make them pregnant

[0051] Four haploid strains of UET1, UET2, UEMT2, and UEMT3 isolated in the laboratory were used to form 16 different strains and combinations of bacteria with different forms (Table 1), and the steps in Example 2 were used to inoculate wild Zizania zizania plants, It was found that only combination (five) and combination (eight) could make the stem base of the plant expand, and there was no obvious difference between the other combinations and the control after inoculation.

[0052] Table 1 Combination list of different strains and different forms of bacteria

[0053]

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Abstract

The invention discloses two pairs of Ustilago esculenta for successful invasion of a zizania aquatica plant and breeding of zizania aquatica and an artificial inoculation method thereof and belongs to the technical field of zizania aquatica artificial inoculation. The two pairs of Ustilago esculenta comprise a pair of an Ustilago esculenta haploid strain UET1 and an Ustilago esculenta haploid strain UET2 and a pair of an Ustilago esculenta haploid strain UET2 and an Ustilago esculenta haploid strain UET3. Wild zizania aquatica is inoculated with the pair of the screened Ustilago esculenta haploid strain UET1 and Ustilago esculenta haploid strain UET2 and the pair of the screened Ustilago esculenta haploid strain UET2 and Ustilago esculenta haploid strain UET3 so that successful breeding of zizania aquatica is realized. The method has simple processes, is effective and provides a novel direction for breeding of zizania aquatica.

Description

technical field [0001] The invention belongs to the field of artificial inoculation of wild rice stems, and in particular relates to two pairs of fungus smut fungi that can successfully infect wild wild wild rice plants and make them pregnant and an artificial inoculation method thereof. Background technique [0002] Ustilago rugosa belongs to the Basidiomycotina genus Ustilago, is a typical dimorphic fungus, and is closely related to Ustilago zea. So far, Zizania is the only known host, and Zizania fertiliser is the result of the interaction between Zizania plants and the smut fungus that specifically parasitizes in the body. So far, the planting and breeding of Zizania zizania still adopts the method of separating and screening Zizania pier, which requires a lot of manpower and material resources. Therefore, artificial inoculation is the development direction of Zizania zizania breeding. But so far there are no reports of successful artificial inoculation. Contents of ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G7/06A01G1/00C12R1/645
CPCA01G7/06A01G22/00C12N1/145C12R2001/645
Inventor 叶子弘崔海峰俞晓平张雅芬
Owner CHINA JILIANG UNIV
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