Bacillus subtilis strain usable as probiotic preparation and fermentation process thereof
A technology of Bacillus subtilis and probiotic preparations, applied in the field of microorganisms, can solve the problems of affecting the micro-ecological balance of livestock and poultry intestines, the increase of drug-resistant pathogenic bacteria, and the excessive drug residues of livestock and poultry products, so as to improve production performance and high-yield enzymes Ability, broad application prospects
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Embodiment 1
[0020] The components of the slant medium are:
[0021] Peptone 5g, glucose 0.5g, agar 15g and perilla seed extract 0.1g.
[0022] The components of the dextrose nutrient broth are:
[0023] Peptone 10g, beef extract 5g, sodium chloride 5g and distilled water 1000ml.
[0024] Step 1: Inoculate Bacillus subtilis on the slant medium, and cultivate it in a 37-degree Celsius incubator for 3 days as a seed culture;
[0025] Step 2: inoculate the seed culture in glucose nutrient broth, culture at 37 degrees Celsius for 24 hours, mix the solution with light calcium carbonate at a ratio of 1:3, dry, and pulverize.
Embodiment 2
[0027] The components of the slant medium are:
[0028] Peptone 10g, glucose 2,5g, agar 15g and perilla seed extract 10g.
[0029] The components of the dextrose nutrient broth are:
[0030] Peptone 10g, beef extract 5g, sodium chloride 5g and distilled water 1000ml.
[0031] Step 1: Inoculate Bacillus subtilis on the slant medium, and cultivate it in a 37-degree Celsius incubator for 3 days as a seed culture;
[0032] Step 2: inoculate the seed culture in glucose nutrient broth, culture at 37 degrees Celsius for 24 hours, mix the solution with light calcium carbonate at a ratio of 1:3, dry, and pulverize.
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