A kind of method and its culture medium for tissue culture and rapid propagation of vaccinium vaccinium

Abstract

The invention discloses a bog bilberry tissue culture fast breeding method and a culture medium thereof. The culture medium for obtaining bog bilberry regeneration plants provided by the invention is prepared from the following components including NH4NO3, Ca(NO3)2.4H2O, KNO3, KH2PO4, MgSO4.7H2O, MnSO4.4H2O, ZnSO4.7H2O, H3BO3, CuSO4.5H2O, NaMoO4.2H2O, phaseomannite, vitaminB1, nicotinic acid, vitaminB6, glycine, Na2-EDTA, FeSO4.7H2O, hormodin, cycocel, sucrose and distilled water. By aiming at the thin and weak phenomena of bog bilberry tissue culture seedlings, the invention provides a method of adding the cycocel into the culture medium to promote the rejuvenation of the bog bilberry tissue culture seedlings in a way of being matched with plant hormone for the first time; the method has the advantages that the adversity ability of the tissue culture seedlings can be enhanced through enhancing the tissue culture seedling lignifications degree, so that the survival rate after the seedling transplanting is improved. Meanwhile, the method provides the auxiliary transplanting by a seedling culture bag. By using the method, the transplanting survival rate of the bog bilberry tissue culture seedling reaches 100 percent.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for tissue culture and rapid propagation of Vaccinium cranberry and its culture medium. Background technique [0002] Vaccinium uliginosum, also known as Dushi, Dianguo, etc., is an edible colored berry of the Ericaceae genus Vaccinium. Its fruit is dark blue, with a delicate taste and a pleasant fruity fragrance. people. Studies have shown that bilberry has anti-oxidation, anti-aging, anti-cancer, cardiovascular disease prevention, vision protection and other effects, and has excellent health care effects. In my country, Vaccinium dusi is concentrated in the Greater Khingan Mountains and the Changbai Mountains, of which the Greater Khingan Mountains is the largest natural distribution area. According to the survey, the natural distribution area of ​​Vaccinium dusi in the Greater Khingan Mountains is over 150,000 hectares, with an annual reserve of about 20,000 tons. ...

AI Technical Summary

Problems solved by technology

However, because the stalks of Vaccinium vaccinium tissue culture seedlings are generally slender, and it is difficult to take root in the bottle, the rooting takes a long time (usually 40-90 days), and the roots are relatively thin

At the same time, there is a lack of research on the transplantation of Vaccinium duskberry tissue culture seedlings, and the survival rate is low.

This situation has invisibly increased the cost of Vaccinium vaccinium seedlings, greatly restricted the process of industrialized seedling cultivation of Vaccinium vaccinium, and restricted the effectiveness of resource protection of Vaccinium vaccinium.

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