A kind of method and its culture medium for tissue culture and rapid propagation of vaccinium vaccinium
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A medium and induction medium technology, applied in the field of plant tissue culture, can solve the problems of weak roots, lack of research, and limitation of bilberry factory seedlings.
Inactive Publication Date: 2018-01-26
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology
However, because the stalks of Vaccinium vaccinium tissue culture seedlings are generally slender, and it is difficult to take root in the bottle, the rooting takes a long time (usually 40-90 days), and the roots are relatively thin
At the same time, there is a lack of research on the transplantation of Vaccinium duskberry tissue culture seedlings, and the survival rate is low.
This situation has invisibly increased the cost of Vaccinium vaccinium seedlings, greatly restricted the process of industrialized seedling cultivation of Vaccinium vaccinium, and restricted the effectiveness of resource protection of Vaccinium vaccinium.
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Embodiment 1
[0041] Embodiment 1, the method for high-efficiency tissue culture, rapid propagation and transplanting of Vaccinium dusi
[0042] 1. Preparation of vaccinium vaccinium induction differentiation medium and rooting medium
[0043] 1. Prepare improved WPM 0 Basal medium:
[0044] Measure 50mL of improved WPM macroelement mother liquor, 5mL of improved WPM trace element mother liquor, 5mL of improved WPM organic matter mother liquor, 5mL of improved WPM iron salt mother liquor, 20mg of sucrose, mix well, adjust the pH to 5.4 with sulfuric acid or phosphoric acid, and dilute the final volume to 1000ml; add 9g of agar, sterilize at 121°C for 20 minutes, let cool to about 65°C, and set aside.
[0045] The improved WPM mother liquor formula prepared by the present embodiment is as shown in Table 1:
[0046] The improved WPM mother liquor formula prepared by the present embodiment of table 1
[0047]
[0048]
[0049] Remarks: All reagents are stored in a 4°C refrigerator af...
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Abstract
The invention discloses a bog bilberry tissue culture fast breeding method and a culture medium thereof. The culture medium for obtaining bog bilberry regeneration plants provided by the invention is prepared from the following components including NH4NO3, Ca(NO3)2.4H2O, KNO3, KH2PO4, MgSO4.7H2O, MnSO4.4H2O, ZnSO4.7H2O, H3BO3, CuSO4.5H2O, NaMoO4.2H2O, phaseomannite, vitaminB1, nicotinic acid, vitaminB6, glycine, Na2-EDTA, FeSO4.7H2O, hormodin, cycocel, sucrose and distilled water. By aiming at the thin and weak phenomena of bog bilberry tissue culture seedlings, the invention provides a method of adding the cycocel into the culture medium to promote the rejuvenation of the bog bilberry tissue culture seedlings in a way of being matched with plant hormone for the first time; the method has the advantages that the adversity ability of the tissue culture seedlings can be enhanced through enhancing the tissue culture seedling lignifications degree, so that the survival rate after the seedling transplanting is improved. Meanwhile, the method provides the auxiliary transplanting by a seedling culture bag. By using the method, the transplanting survival rate of the bog bilberry tissue culture seedling reaches 100 percent.
Description
technical field [0001] The invention relates to plant tissue culture technology, in particular to a method for tissue culture and rapid propagation of Vaccinium cranberry and its culture medium. Background technique [0002] Vaccinium uliginosum, also known as Dushi, Dianguo, etc., is an edible colored berry of the Ericaceae genus Vaccinium. Its fruit is dark blue, with a delicate taste and a pleasant fruity fragrance. people. Studies have shown that bilberry has anti-oxidation, anti-aging, anti-cancer, cardiovascular disease prevention, vision protection and other effects, and has excellent health care effects. In my country, Vaccinium dusi is concentrated in the Greater Khingan Mountains and the Changbai Mountains, of which the Greater Khingan Mountains is the largest natural distribution area. According to the survey, the natural distribution area of Vaccinium dusi in the Greater Khingan Mountains is over 150,000 hectares, with an annual reserve of about 20,000 tons. ...
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