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Preparation method and application of acremonium strictum of endophytic fungi of panax notoginseng

An endophytic fungus and Acremonium spp. technology, applied in the field of bioengineering, can solve the problems of research on endophytic fungi of Panax notoginseng, and the related application has not been reported, and achieves reduction in the use of pesticides and fertilizers, economical and effective reagents, and convenient operation. Effect

Inactive Publication Date: 2016-08-31
杜云龙
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Panax notoginseng is a kind of traditional Chinese medicine, which has the effects of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain, but the research and related application of Sanqi endophytic fungi have not been reported yet.

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  • Preparation method and application of acremonium strictum of endophytic fungi of panax notoginseng
  • Preparation method and application of acremonium strictum of endophytic fungi of panax notoginseng
  • Preparation method and application of acremonium strictum of endophytic fungi of panax notoginseng

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Embodiment Construction

[0028] The preparation method and application of a kind of notoginseng endophytic fungus Acremonium acremonium of the present invention will be further described in detail in conjunction with specific examples below.

[0029] The preparation of a kind of Panax notoginseng endophyte acremonium acremonium of the present invention:

[0030] Cut off the dormant buds from the root of Panax notoginseng, wash the surface with clean water, then soak in 70% ethanol for 1.5 minutes, and wash 3 times with sterile water; put the dormant buds into a solution containing 10% sodium hypochlorite, and disinfect the surface for 10 minutes , and then washed 3 times with sterile water; then put the dormant buds into the Erlenmeyer flask containing MS medium, and after 1 month of light culture, the mycelium grew. Single spores were picked under a light microscope and cultured in PDA medium.

[0031] When the diameter of the colony grows to 4 cm, pick a part of mycelia and spores for morphological...

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Abstract

The invention provides a preparation method and application of acremonium strictum of endophytic fungi of panax notoginseng. The method comprises the steps of cutting off dormant buds from a root tuber of the panax notoginseng, cleaning the surfaces of the dormant buds with clear water, then soaking the cleaned dormant buds into 70 percent alcohol for 1.5 minutes, and washing the dormant buds with sterile water for three times; putting the dormant buds into a solution containing 10 percent sodium hypochlorite, sterilizing the surfaces of the dormant buds for 10 minutes, and then washing the dormant buds with the sterile water for three times; putting the dormant buds into a conical flask comprising an MS culture medium, and culturing for 1 month under the condition of illumination to obtain hyphae growing in the dormant buds; picking a single spore under an optical microscope, and culturing the picked single spore in a PDA culture medium. The acremonium strictum of the endophytic fungi of the panax notoginseng is capable of promoting the germination of the panax notoginseng and the development of a root system.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a preparation method of the notoginseng endophytic fungus Acremonium acremonium and its application. Background technique [0002] Endophytic fungi are a special type of fungi that grow in healthy living plant tissues. They are normal flora in plant tissues, mainly in tissues below the surface cell layer, and the host tissues are temporarily asymptomatic. Endophytic fungi coexist with their hosts for a long time, so there may be a genetic system that can exchange genetic information with plant hosts, have unique biological and genetic characteristics, and can produce new compounds with various biological activities, such as antibiotics, plant Growth regulators, immunosuppressive active substances, antitumor active substances, etc. The content of active substances derived from plants is generally very low, and the plant resources in the world are decreasing day by day, so the cost o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00A01G7/06A01C1/00C12R1/645
CPCA01C1/00A01G7/06A01N63/30C12N1/145C12R2001/645
Inventor 杜云龙
Owner 杜云龙