Preparation method and application of acremonium strictum of endophytic fungi of panax notoginseng
An endophytic fungus and Acremonium spp. technology, applied in the field of bioengineering, can solve the problems of research on endophytic fungi of Panax notoginseng, and the related application has not been reported, and achieves reduction in the use of pesticides and fertilizers, economical and effective reagents, and convenient operation. Effect
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[0028] The preparation method and application of a kind of notoginseng endophytic fungus Acremonium acremonium of the present invention will be further described in detail in conjunction with specific examples below.
[0029] The preparation of a kind of Panax notoginseng endophyte acremonium acremonium of the present invention:
[0030] Cut off the dormant buds from the root of Panax notoginseng, wash the surface with clean water, then soak in 70% ethanol for 1.5 minutes, and wash 3 times with sterile water; put the dormant buds into a solution containing 10% sodium hypochlorite, and disinfect the surface for 10 minutes , and then washed 3 times with sterile water; then put the dormant buds into the Erlenmeyer flask containing MS medium, and after 1 month of light culture, the mycelium grew. Single spores were picked under a light microscope and cultured in PDA medium.
[0031] When the diameter of the colony grows to 4 cm, pick a part of mycelia and spores for morphological...
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