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Microcapsules and fat powder containing β-carotene

一种胡萝卜素、微胶囊的技术，应用在微生物、微生物、食品的成形等方向，能够解决β-胡萝卜素产量低等问题，达到发酵工艺简单、利于工业化生产、便于控制的效果

Active Publication Date: 2019-10-15
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of β-carotene produced by using this strain is low, and the highest yield does not exceed 1.3g / L

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] In order to understand the technical content of the present invention more clearly, the following examples are given in detail.

[0010] Breeding method of mutant strain of B. trispora

[0011] 1) Commercially available B. trispora positive bacteria (preservation number: CCTCCAF97006(+)) and B. trispora negative bacteria (preservation number: CCTCCAF96002(-)) were used as starting strains.

[0012] 2) The two starting strains were cultured for 5 days until the spores matured.

[0013] 3) Filter through gauze or filter paper to obtain pure spore liquid, inject the spore liquid into a sterile petri dish, and dry it with sterile air to become bacterial plaque. Aseptically move the petri dish containing bacterial plaque into a high-energy particle beam implanter, and undergo mutagenesis by high-energy ion beam implantation.

[0014] 4) The bacterial plaques of the positive and negative strains were eluted with sterile water respectively, and the obtained positive and neg...

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PUM

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Abstract

The present invention provides microcapsules or fat powder containing β-carotene, the β-carotene is obtained by fermentation of a mutant strain of B. trispora, characterized in that: the mutant strain of B. trispora was produced on August 2014 It was deposited in the China Center for Type Culture Collection (CCTCC) on March 8, and the deposit address is Wuhan University, Wuhan, China. ‑) CCTCC M 2014379. The B. trispora mutant strain of the present invention can obtain higher yield of β-carotene after conventional fermentation and cultivation. Simultaneously, the whole fermentation process is simple, easy to control and beneficial to industrialized production.

Description

technical field [0001] The invention relates to a microcapsule containing beta-carotene and fat powder. Background technique [0002] β-carotene is one of more than 500 kinds of carotene. It is the precursor of vitamin A, also known as provitamin A. Its dilute solution is orange-yellow to yellow. β-carotene widely exists in plants, algae and fungi, but animals and humans cannot synthesize carotene and must be ingested from the outside. As a food additive, β-carotene can act as a pigment and a nutritional enhancer. At the same time, β-carotene is a powerful antioxidant, which can inhibit the proliferation of cancer cells and improve the body's immunity. The unique nutritional value and medicinal efficacy make carotene highly valued by medical circles and food merchants all over the world. [0003] At present, the preparation methods of β-carotene mainly include the following types: 1. Chemical synthesis, that is, using organic chemical raw materials to artificially synthesi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00A23L33/10A23P10/30C12R1/645
CPCA23V2002/00C12P23/00C12N1/145C12R2001/645A23V2250/211
Inventor 汪志明李翔宇余超陆姝欢周强肖敏张玉良
Owner CABIO BIOTECH WUHAN CO LTD