Unlock instant, AI-driven research and patent intelligence for your innovation.

Dual specific binding proteins directed against immune cell receptors and tlr signaling autoantigens

A binding protein, bispecific technology, applied in immunoglobulin, anti-animal/human immunoglobulin, anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, etc.

Inactive Publication Date: 2016-09-07
ABBVIE INC +1
View PDF18 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, initial attempts at using F(ab')2 anti-mouse IgM (anti-IgM) to cross-link BCR and CpG DNA to stimulate TLR-9 replication to effect the linking of BCR to TLR9 have not been replicated with spontaneous immune complexes All aspects of stimulation (Chaturvedi et al. (2008) Immunity 28(6):799-809)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dual specific binding proteins directed against immune cell receptors and tlr signaling autoantigens
  • Dual specific binding proteins directed against immune cell receptors and tlr signaling autoantigens
  • Dual specific binding proteins directed against immune cell receptors and tlr signaling autoantigens

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0212] Example 1: Anti-mouse IgM and anti-DNA dual variable domain immunoglobulin (DVD-Ig TM ) protein generation and characterization

[0213] Four-chain dual variable domain immunoglobulin (DVD -Ig TM )protein. DVD-Ig TM The constructs were cloned and expressed in human embryonic kidney 293 cells and purified according to methods known in the art. for DVD-Ig TM The VH and VL chain amino acid sequences of the proteins are provided in Table 1. The SEQ ID NO listed in the leftmost column of Table 2 refers to the DVD-Ig in that row of the table TM The sequence of the complete variable domains of the heavy and light chains of the protein. Each row in the rightmost column of Table 2 provides three SEQ ID NOs. The first number refers to the SEQ ID NO of the outer variable domain sequence, the second number refers to the SEQ ID NO of the linker, and the third number refers to the SEQ ID NO of the inner variable domain sequence, which are common in a complete DVD-Ig TM Varia...

example 2

[0221] Example 2: For the determination of parental antibodies and DVD-Ig TM Determination of functional activity of proteins

example 21

[0222] Example 2.1: Mice

[0223] AM14 B cell receptor (BCR) transgenic mice have been described previously (Sweet et al. (2010) Autoimmun. 43(8):607-18). Briefly, AM14 is a BCR comprising AM14 heavy chain and Vk8 light chain that recognizes the "a" allotype of murine IgG2a (mIgG2a). 95-98% of B cells in mice positive for AM14 heavy chain and Vk8 light chain express AM14BCR. To generate AM14B cells deficient in TLR9, TLR7 or FcyRIIB, AM14 and Vk8 genes were bred to appropriate knockout mice.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Engineered multivalent and multispecific binding proteins that bind immune cell receptors and / or auto antigens are provided, along with methods of making and uses in the prevention, diagnosis, prognosis and / or treatment of disease.

Description

[0001] related application [0002] This patent application claims priority to U.S. Provisional Patent Application No. 61 / 887,412, filed October 6, 2013, and U.S. Provisional Patent Application No. 61 / 987,587, filed May 2, 2014, both U.S. Provisional Both patent applications are incorporated herein by reference in their entirety. technical field [0003] The present invention provides multivalent and multispecific binding proteins that bind to B cell receptors and self-antigens, methods of preparation and uses thereof, including the diagnosis, prognosis, prevention and treatment of autoimmune diseases, as well as the identification of therapeutic agents and clinical trial candidates filter. Background technique [0004] Autoimmune diseases are common health problems, however, the etiology of these diseases remains poorly understood. Autoimmune diseases can be divided into two broad but overlapping categories: organ-specific and systemic. In organ-specific autoimmune dise...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C07K16/44C07K16/18C07K16/42
CPCC07K16/18C07K16/2803C07K16/4283C07K16/44C07K2317/31A61K47/6849A61P13/12A61P19/02A61P21/00A61P25/00A61P29/00A61P37/02A61P43/00A61K39/39533A61K39/3955A61K45/06A61K49/0058C07K16/28C07K16/2866C07K2317/74C07K2319/80C07K2319/85G01N33/5052G01N33/564G01N2333/705G01N2500/10G01N2800/52
Inventor 崔齐浩塔里克·加哈尤里安·马沙克-罗斯坦克里希那·莫迪
Owner ABBVIE INC