Nerve cells protection method
A nerve cell protection and nerve cell technology, applied in the field of biomedical engineering, can solve problems such as unreported, and achieve the effects of low price, wide clinical application prospects, and simple operation.
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[0022] Embodiment 1: Nerve cell protection experiment of plasma
[0023] 1. Experimental materials
[0024] 1. SH-SY5Y cells were purchased from the Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences.
[0025] 2. 1640 medium and FBS (Fetal Bovine Serum) were purchased from Gibco.
[0026] 3. Plasma device: Provided by Beijing Institute of Technology, it is a coaxial coplanar DBD discharge device. The inner diameter of the dielectric tube is 1mm, the outer diameter is 2mm, and the two electrodes are wound by metal aluminum foil with a width of 3mm. The left electrode is a power electrode , the driving power is an alternating current signal with a frequency of f=30kHz, the electrode on the right is a ground electrode, and high-purity helium flows in from the left end of the dielectric tube at a fixed flow rate.
[0027] 4. CCK-8 cell proliferation and activity detection kit: purchased from Tongren Institute of Chemistry (CK04).
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