Inflammation diminishing and acne removing mask and preparation method thereof
A mask and acne-removing technology, which is applied to the field of anti-inflammatory and acne-removing masks and their preparation, can solve the problems of infected animals, affecting the acne-removing effect, and low absorption efficiency of the mask, and achieves the effect of high absorption efficiency.
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[0054] The invention provides a preparation method of anti-inflammatory and acne-removing facial mask, comprising:
[0055] Mix honeysuckle extract, aloe vera extract, pearl powder, stem cell growth factor powder and hydrogel to obtain a mask liquid;
[0056] The mask liquid is prepared into a mask to obtain an anti-inflammatory and acne-removing mask.
[0057] In the present invention, the honeysuckle extract and the aloe extract are preferably dissolved in water to obtain a mixed liquid; the stem cell growth factor powder and pearl powder are dissolved in the mixed liquid to obtain a mixture; the mixture is mixed with hydrogel to obtain a mask liquid. In the present invention, the mixture and the hydrogel are preferably mixed in an ice bath to obtain a mask liquid. In the present invention, the mass concentration of honeysuckle extract in the mixed solution is preferably 2 mg / mL-10 mg / mL, more preferably 4 mg / mL-8 mg / mL, most preferably 5 mg / mL-6 mg / mL. In the present inve...
Embodiment 1
[0066] The serum-free medium containing mesenchymal stem cells was inoculated on a petri dish for cell culture at a seeding density of 1.0×10 5 cell / ml;
[0067] Replace the culture medium once every 3 days, subculture with more than 80% confluence, and collect the culture medium of the 3rd to 5th generation cells;
[0068] Filter the obtained cell culture solution through a 0.45 μm filter membrane to remove large particles, then filter the obtained filtrate through a 0.22 μm filter membrane to remove small particles below 3KDa, and use a Slice200 tangential flow device to filter the obtained filtrate for 3 hours Concentration to obtain a concentration solution with a protein mass concentration of 1000mg / L;
[0069] The obtained concentrate is frozen on a lyophilizer to obtain stem cell growth factor powder.
Embodiment 2
[0071] Dissolve polyvinyl alcohol (mPEG) in a mixed solution of dimethyl sulfoxide (DMSO) and dichloromethane (CF) (volume ratio V(DMSO) / V(CF)=10, molar ratio n(DMSO) / n (mPEG)=75), drip acetic anhydride in above-mentioned polyvinyl alcohol solution under nitrogen environment; Dissolve with dichloromethane after the mixture that obtains is precipitated with ether, repeat 2 times of precipitation and dissolving; Vacuum the product after dissolving Dried to obtain an intermediate product;
[0072] Chitosan was dissolved in a mixed aqueous solution of acetic acid and methanol (the mass concentration of acetic acid in the mixed aqueous solution was 0.1 mol / L). Dissolve the above intermediate product in deionized water to form a solution, drop this solution into the above chitosan solution, adjust the pH to 6.0 with NaOH, and then add NaBH dropwise to it 3 CN solution (NaBH 3 The mass ratio of CN to the above-mentioned intermediate product is 1 / 40), stirred for 18h to react; the o...
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