A kind of tissue culture method of rapid detoxification of purple potato

Abstract

The invention provides a tissue culture method for rapid detoxification of purple potatoes. The method includes the following steps that 1, bud stems of the purple potatoes are cut; 2, the cut bud stems are subjected to sterile water cleaning, instantaneous alcohol disinfection, disinfectant soaking disinfection and sterile water washing in sequence, and disinfected bud stems are obtained; 3, the disinfected bud stems are placed on an ultra-clean bench, stem tips at the top ends of the bud stems are stripped under a microscope and then inoculated in a culture medium for primary tissue culture, and primary test-tube plantlets are obtained; 4, stem tips of the primary test-tube plantlets are stripped, secondary tissue culture is performed, and secondary test-tube plantlets are obtained; 5, stripping and tissue culture in the step 4 are repeatedly preformed 3-5 times, and detoxified test-tube plantlets are obtained. The detoxification rate of the purple potatoes cultured with the method reaches up to 93%, the reproduction cycle is shortened by 2-4 months, batched and rapid reproduction of the purple potatoes can be achieved, and the culture cost can be lowered.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture method for rapid detoxification of purple potatoes. Background technique [0002] Purple potato, the shape of the potato is oblong, the bud eyes are small, the flesh is dark purple, rich in natural pigments such as anthocyanins. Purple potatoes have a good-looking appearance and strong color allure. There is no need to add any pigments and color enhancers during the product processing, which is extremely healthy. [0003] The origin of purple potatoes is Peru and other countries in South America. At present, some varieties of purple potatoes have been introduced by units and individuals in my country. However, due to the fact that different purple potatoes are planted in different regions, after planting, the varieties in different regions are planted in the same plot, area, quantity, and single weight. There are great differences in each variety. ...

AI Technical Summary

Problems solved by technology

However, due to the fact that different purple potatoes are introduced in different regions during the planting process, after planting, the varieties in different regions are planted in the same plot, area, quantity, and monomer weight. There are great differences in each variety.

One is that the plant growth declines and the compound leaves shrink; the other is that the plant type becomes shorter and the leaves shrink; the third is that the leaves appear yellow and green spots, the veins are necrotic, and the compound leaves fall off, etc. Some imported varieties are planted for the first time. High yield per hour, low yield after the second planting after reserved seeds, and serious degradation of small tubers; purple potato cannot grow stably for a long time after introduction, especially the offspring of reserved seeds cannot maintain the original performance and yield of plants

The reason is that after the introduction, in the process of planting in the planting area, it is infected by germs, viruses, etc. However, there is no mature and effective purple potato virus-free cultivation method in the prior art.

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