Preparation method of polymer micelles loaded with photosensitizer and application of the micelles in killing planktonic bacteria and bacterial biofilms

A technology of planktonic bacteria and polymers, applied in the field of nano-biomedical materials, can solve the problems of limiting biological distribution and production efficiency of active oxygen components, low water solubility, etc., achieve good singlet oxygen production efficiency, low light energy, and easy conversion Effect

Active Publication Date: 2018-11-13
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest obstacle encountered in the clinical application of protoporphyrin-based photosensitizers is their low water solubility (water solubility of 1 μg mL at 25 °C –1 ), which greatly limits its biodistribution and production efficiency of reactive oxygen species

Method used

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  • Preparation method of polymer micelles loaded with photosensitizer and application of the micelles in killing planktonic bacteria and bacterial biofilms
  • Preparation method of polymer micelles loaded with photosensitizer and application of the micelles in killing planktonic bacteria and bacterial biofilms
  • Preparation method of polymer micelles loaded with photosensitizer and application of the micelles in killing planktonic bacteria and bacterial biofilms

Examples

Experimental program
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Effect test

Embodiment

[0034] (1) The preparation of polyethylene glycol-b-polycyclocaprolactone (PEG-b-PCL), the steps are as follows:

[0035] 1) Add 2.0g CH 3 O-PEG 114 -OH (0.4 mmol, PDI: 1.04) and 4.2 g of redistilled ε-CL (36.8 mmol, 92 eq.) were added to a 50 mL dry Schlenk bottle.

[0036] 2) Add 15mL double-distilled anhydrous toluene to dissolve, add a drop of Sn(Oct) 2 (0.5 mol%).

[0037] 3) Freeze with liquid nitrogen—vacuumize—fill with nitrogen—thaw, and cycle three times.

[0038] 4) React overnight in an oil bath at 100° C. under nitrogen protection.

[0039] 5) After the reaction is completed, cool to room temperature, add an appropriate amount of dichloromethane to dilute, and then precipitate in glacial ether. After the precipitation is complete, filter with suction, wash the filter cake three times with glacial ether, and dry the white powdery solid obtained after vacuum drying, which is PEG 5k -b-PCL.

[0040] (2) Preparation of polycyclocaprolactone-b-poly-β-urethane (P...

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Abstract

The invention discloses a preparation method of photosensitizer-supported polymeric micelle and an application of the micelle in killing of planktobacteria and bacterial biofilms. The photosensitizer-supported polymeric micelle is prepared from two block polymers PEG-b-PCL and PCL-b-PAE and is used for killing multiple drug-resistant bacteria and biofilms. A photosensitizer supported on composite shell micelle has good sterilization efficiency on bioluminescence gene transfected staphylococcus aureus S.aureus Xen36 drug-resistant to kanamycin and drug-resistant bacteria in the biofilms. A micellar system has the advantages as follows: 1) the preparation is simple; 2) a light source is simple, a complicated controller like a laser is not required, and light energy is very low and is easily converted to the clinical direction; 3) good singlet oxygen production efficiency is realized; 4) the photosensitizer-supported polymeric micelle has good killing effects on the drug-resistant bacteria and the biofilms and has good application prospect.

Description

technical field [0001] The invention belongs to the field of nano biomedical materials, and relates to a method for killing multidrug-resistant bacteria and biofilms by polymer micelles loaded with photosensitizers. Background technique [0002] Since the accidental discovery of penicillin in 1929 to treat Gram-positive pathogenic bacteria Streptococcus and Staphylococcus and the discovery in 1943 that streptomycin can inhibit Mycobacterium tuberculosis, antibiotics have opened its golden age. In the ensuing decades, new antibiotics and evolving methods of derivative synthesis rounded out the antibiotic library. Unfortunately, bacterial resistance has existed since ancient times. Over the past three decades, the development of pathogenic bacteria's resistance to antibiotics has been far faster than the discovery of new antibiotics. The cause of bacterial resistance is mainly due to the misuse of antibiotics, especially in China. In 2009, 60% of Staphylococcus aureus isola...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01N25/10A01P1/00
CPCA01N25/10A01N43/90A01N2300/00
Inventor 史林启刘勇张珍坤亨克﹒卜歇儿亨利﹒万德梅任艺瑾
Owner NANKAI UNIV
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