Thauera bacterial strain and application thereof

Abstract

The invention discloses thauera bacterial strain and application thereof and belongs to the field of environmental microorganism application. The bacterial strain is named as Thauera sp. CM-HZX2, the bacterial strain is preserved in China Center for Type Culture Collection (CCTCC) at present, the preservation number is CCTCC NO:M2015689, and the preservation data is November 2015. The thauera bacterial strain CM-HZX2 is high-efficient flocculating agent producing strain and can be widely applied to domestic wastewater, especially high-turbidity wastewater and a polluted water body of raw water. The thauera bacterial strain can grow best in conditions of pH of 7.0, temperature of 30 DEG C and rotation speed of 150r / min. After performing liquid shake culturing for 72h, the flocculation rate can reach up to 92.3%.

Description

technical field

[0001] The invention relates to the application field of environmental microorganisms, in particular to a Taueria strain and its application. Background technique

[0002] Flocculants are mainly divided into inorganic flocculants, organic polymer flocculants and microbial flocculants. Among them, inorganic flocculants occupy an important share in my country's current market, with an annual demand of more than 100,000 tons, mainly represented by iron salts and aluminum salts.

[0003] Among them, iron salts are corrosive and easy to form residues, which will color the treated water and cause secondary pollution; in hygiene, it has been found that the accumulation of aluminum salts in the human body is directly related to today's Alzheimer's disease. Macromolecular organic flocculants, such as polyacrylamide, are not toxic in themselves, but are not easy to decompose. Long-term accumulation in nature will also cause secondary pollution. The acrylamide monomer ...

Examples

Embodiment 1

[0035] Example 1 Isolation and Identification of Tauerella sp. CM-HZX2

[0036] 1. Isolation of Thauera sp. CM-HZX2

[0037] Thauera sp. CM-HZX2 was isolated from the activated sludge in the aerobic tank of Huzhou sewage treatment plant.

[0038] (1) culture medium

[0039] LB medium: tryptone: 10g L -1 , Yeast powder: 5g·L -1 , NaCl: 10g·L -1 , prepared with phenol-free distilled water, adjusted the pH value to 7.0, and sterilized by high-temperature steam at 121°C for 20 minutes. 10-20g·L should be added to the LB agar plate medium -1 of agar.

[0040] Fermentation medium: Glucose: 6.0g·L -1 , yeast powder: 4.0g·L -1 , K2 HPO 4 : 5.0g·L -1 , KH 2 PO 4 : 2.0g·L -1 , NaCl: 0.1g·L -1 , MgSO 4 : 0.2g·L -1 , prepared with distilled water, adjusted to a pH value of 7.0, and sterilized by high-temperature steam at 115°C for 20 minutes.

[0041] (2) Isolation and purification of bacterial strains

[0042] Take an appropriate amount of activated sludge samples (abou...

Embodiment 2

[0063] The optimum growth condition optimization of embodiment 2 Taoerella (Thauera sp.) CM-HZX2

[0064] Medium: LB medium Tryptone: 10g·L -1 , Yeast powder: 5g·L -1 , NaCl: 10g·L -1 , prepared with phenol-free distilled water, adjusted the pH value to 7.0, and sterilized by high-temperature steam at 121°C for 20 minutes.

[0065] (1) Effect of the initial pH value of the medium on the growth activity of Thauera sp. CM-HZX2

[0066] The effect of initial pH on the growth activity of Thauera sp. CM-HZX2 was investigated by changing the pH value in the medium. Adjust the initial pH of the culture medium to 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, shake culture at 30°C and 150r / min for 72 hours, measure the growth of bacteria .) CM-HZX2 is used as a blank comparison, and the OD600nm value is detected by sampling at certain intervals.

[0067] From image 3 It can be seen that the bacterium has a very low absorbance value and slow growth in the medium whose initial pH is below 5...

Embodiment 3

[0074] The salt resistance of embodiment 3 Taoerella (Thauera sp.) CM-HZX2

[0075] In complex industrial wastewater, the salt content is relatively high. By changing the salt content in the culture medium, the salt resistance ability of Tauerella strains was investigated. The LB medium was prepared, the transfer volume was about 5%, the initial pH value was 7, cultured on a shaker at 30°C and 150r / min, and samples were taken at certain intervals to detect the OD 600nm value.

[0076] see the results below Figure 6 , when the concentration is 0-4%, with the increase of salt concentration, the OD 600nm of microbial growth is stable, and at 5% salinity, the growth absorbance value decreases, indicating that CM-HZX2 belongs to moderate salt-tolerant bacteria.

technical field

[0001] The invention relates to the application field of environmental microorganisms, in particular to a Taueria strain and its application. Background technique

[0002] Flocculants are mainly divided into inorganic flocculants, organic polymer flocculants and microbial flocculants. Among them, inorganic flocculants occupy an important share in my country's current market, with an annual demand of more than 100,000 tons, mainly represented by iron salts and aluminum salts.

[0003] Among them, iron salts are corrosive and easy to form residues, which will color the treated water and cause secondary pollution; in hygiene, it has been found that the accumulation of aluminum salts in the human body is directly related to today's Alzheimer's disease. Macromolecular organic flocculants, such as polyacrylamide, are not toxic in themselves, but are not easy to decompose. Long-term accumulation in nature will also cause secondary pollution. The acrylamide monomer ...

Examples

Embodiment 1

[0035] Example 1 Isolation and Identification of Tauerella sp. CM-HZX2

[0036] 1. Isolation of Thauera sp. CM-HZX2

[0037] Thauera sp. CM-HZX2 was isolated from the activated sludge in the aerobic tank of Huzhou sewage treatment plant.

[0038] (1) culture medium

[0039] LB medium: tryptone: 10g L -1 , Yeast powder: 5g·L -1 , NaCl: 10g·L -1 , prepared with phenol-free distilled water, adjusted the pH value to 7.0, and sterilized by high-temperature steam at 121°C for 20 minutes. 10-20g·L should be added to the LB agar plate medium -1 of agar.

[0040] Fermentation medium: Glucose: 6.0g·L -1 , yeast powder: 4.0g·L -1 , K2 HPO 4 : 5.0g·L -1 , KH 2 PO 4 : 2.0g·L -1 , NaCl: 0.1g·L -1 , MgSO 4 : 0.2g·L -1 , prepared with distilled water, adjusted to a pH value of 7.0, and sterilized by high-temperature steam at 115°C for 20 minutes.

[0041] (2) Isolation and purification of bacterial strains

[0042] Take an appropriate amount of activated sludge samples (abou...

Embodiment 2

[0063] The optimum growth condition optimization of embodiment 2 Taoerella (Thauera sp.) CM-HZX2

[0064] Medium: LB medium Tryptone: 10g·L -1 , Yeast powder: 5g·L -1 , NaCl: 10g·L -1 , prepared with phenol-free distilled water, adjusted the pH value to 7.0, and sterilized by high-temperature steam at 121°C for 20 minutes.

[0065] (1) Effect of the initial pH value of the medium on the growth activity of Thauera sp. CM-HZX2

[0066] The effect of initial pH on the growth activity of Thauera sp. CM-HZX2 was investigated by changing the pH value in the medium. Adjust the initial pH of the culture medium to 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, shake culture at 30°C and 150r / min for 72 hours, measure the growth of bacteria .) CM-HZX2 is used as a blank comparison, and the OD600nm value is detected by sampling at certain intervals.

[0067] From image 3 It can be seen that the bacterium has a very low absorbance value and slow growth in the medium whose initial pH is below 5...

Embodiment 3

[0074] The salt resistance of embodiment 3 Taoerella (Thauera sp.) CM-HZX2

[0075] In complex industrial wastewater, the salt content is relatively high. By changing the salt content in the culture medium, the salt resistance ability of Tauerella strains was investigated. The LB medium was prepared, the transfer volume was about 5%, the initial pH value was 7, cultured on a shaker at 30°C and 150r / min, and samples were taken at certain intervals to detect the OD 600nm value.

[0076] see the results below Figure 6 , when the concentration is 0-4%, with the increase of salt concentration, the OD 600nm of microbial growth is stable, and at 5% salinity, the growth absorbance value decreases, indicating that CM-HZX2 belongs to moderate salt-tolerant bacteria.

Images