A kit for measuring 1,5-anhydroglucitol and a preparing method thereof

An anhydroglucitol and kit technology, applied in the fields of medicine and biochemistry, can solve the problems of complex operation and poor detection accuracy, and achieve the effect of accurate detection results, simple operation, and elimination of interference

Inactive Publication Date: 2016-10-26
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a test kit for measuring 1,5-anhydroglucitol and its preparation method in order to overcome the defects of complicated operation and poor detection accuracy of existing detection methods

Method used

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  • A kit for measuring 1,5-anhydroglucitol and a preparing method thereof
  • A kit for measuring 1,5-anhydroglucitol and a preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0076] Reagent R1:

[0077] Tris buffer 100 mmol / L

[0078]Glucokinase 35 g / L

[0079] Pyruvate Kinase 3.5 KU / L

[0080] Ascorbate Oxidase 6.0 KU / L

[0081] Adenosine triphosphate 1.0 mmol / L

[0082] Enol Phosphate Pyruvate 4.0 mmol / L

[0083] 4-Aminoantipyridine 1.0 mmol / L

[0084] MgCl2 15 mmol / L

[0085] KCl 80mmol / L

[0086] Its solvent is purified water.

[0087] Reagent R2:

[0088] Tris buffer 100 mmol / L

[0089] Pyranose oxidase 100 KU / L

[0090] Horseradish peroxidase 8 KU / L

[0091] 3-Hydroxy-2,4,6-triiodobenzoic acid 4.5 mmol / L

[0092] Its solvent is purified water.

Embodiment 2

[0094] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0095] Reagent R1:

[0096] Tris buffer 110 mmol / L

[0097] Glucokinase 65 g / L

[0098] Pyruvate Kinase 7.0 KU / L

[0099] Ascorbate oxidase 2.0KU / L

[0100] Adenosine triphosphate 0.2 mmol / L

[0101] Enol Phosphate Pyruvate 6.5 mmol / L

[0102] 4-Aminoantipyridine 0.2 mmol / L

[0103] MgCl2 5 mmol / L

[0104] KCl 10mmol / L

[0105] Its solvent is purified water.

[0106] Reagent R2:

[0107] Tris buffer 20mmol / L

[0108] Pyranose oxidase 150 KU / L

[0109] Horseradish Peroxidase 16 KU / L

[0110] 3-Hydroxy-2,4,6-triiodobenzoic acid 0.5mmol / L

[0111] Its solvent is purified water.

Embodiment 3

[0113] Kit preparation and method of use

[0114] 1. Prepare the reagent according to the content of the following components:

[0115] Reagent R1:

[0116] Tris buffer 100 mmol / L

[0117] Glucokinase 35 g / L

[0118] Pyruvate Kinase 3.5 KU / L

[0119] Ascorbate Oxidase 6.0 KU / L

[0120] Adenosine triphosphate 1.0 mmol / L

[0121] Enol Phosphate Pyruvate 4.0 mmol / L

[0122] 4-Aminoantipyridine 1.0 mmol / L

[0123] MgCl2 15 mmol / L

[0124] KCl 80mmol / L

[0125] Its solvent is purified water.

[0126] Reagent R2:

[0127] Tris buffer 100 mmol / L

[0128] Pyranose oxidase 100 KU / L

[0129] Horseradish peroxidase 8 KU / L

[0130] 3-Hydroxy-2,4,6-triiodobenzoic acid 4.5 mmol / L

[0131] Its solvent is purified water.

[0132] 2. Parameter setting of automatic biochemical analyzer

[0133] (a) Detection temperature: 37°C;

[0134] (b) Detection wavelength: main wavelength 570nm, secondary wavelength 700nm;

[0135] (c) Reaction time: 10 minutes, among them, the incubation...

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Abstract

A kit for measuring 1,5-anhydroglucitol and a preparing method thereof are disclosed. The kit comprises a reagent R1 and a reagent R2 which are independent two liquid components. The reagent R1 comprises a Tris buffer liquid, glucokinase, pyruvate kinase, ascorbic acid oxidase, adenosine triphosphate, phosphoenolpyruvate, 4-aminoantipyrine, MgCl2 and KCl, and a solvent of the reagent R1 is purified water. The reagent R2 comprises a Tris buffer liquid, pyranose oxidase, horse radish peroxidase and 3-hydroxy-2,4,6-triiodobenzoic acid, and a solvent of the reagent R2 is purified water. The preparing method includes mixing a serum sample to be measured with the reagent R1 and the reagent R2, measuring an absorbance difference after a reaction, and calculating the concentration of the 1,5-anhydroglucitol in the sample. The kit and the method have advantages of high accuracy, and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring 1,5-anhydroglucitol and a preparation method thereof. Background technique [0002] 1,5-anhydroglucitol (1,5-AG), also known as 1,5-anhydrosorbitol or 1-deoxyglucose, is a six-carbon monosaccharide with a pyran ring structure, widely distributed in various tissues of the human body Among them, the content of 1,5-AG in other tissues far exceeds that in the blood, and because of the slow metabolism, the concentration in the blood changes little. It exists in unbound form, with small molecular weight and strong hydrophilicity. It can freely pass through the glomerular basement membrane and is almost completely reabsorbed in the renal tubules. This reabsorption is obviously inhibited by urinary glucose excretion. [0003] The level of 1,5-anhydroglucitol (1,5-AG) in plasma is significantly negatively correlated with glucose, glycosylated hemogl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/28C12Q1/26
CPCC12Q1/26C12Q1/28C12Q1/48
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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