Method for improving raising rate of triploid of bream and gurnard

Abstract

The invention discloses a method for improving raising rate of triploid of bream and gurnard. The method comprises the following steps: preparing a tamoxifen inhibitor, preparing a tamoxifen injection, injecting to treat female parents, performing cold shock and hydrostatic pressure treatment, preparing an estrogen inhibitor solution, obtaining a bream and gurnard male tetraploid pure line, and obtaining a triploid by using a backcross method. Through the method, a 100% bream and gurnard triploid pure line can be obtained. Compared with hybridization, the method can obtain the triploid pure line which is higher in purity and more stable in later generations. Compared with the prior art, troubles of breeding and seed selection are reduced. More importantly, the method has no pollution on natural aquatic product germplasm and ecological environment.

Description

technical field [0001] The invention relates to a triploid breeding method for bream and bream, in particular to a method for increasing the triploid breeding rate of bream and bream. Background technique [0002] Bream is also called Changchun bream, grass bream, etc. Bream is divided into triangular bream and round head bream. It grows faster, has strong adaptability, has a wide appetite, tender meat, and delicious taste. It is one of the main freshwater cultured fish in China. . The sterility characteristics of triploid bream and bream make them show more significant advantages in growth speed, stress resistance, disease resistance and meat quality. In the prior art, sterile triploid fish are obtained by crossing between tetraploid fish and diploid fish, but to obtain more than 98% triploid fish, it is necessary to establish genetically stable and extremely high-purity Tetraploid fish. [0003] In fish genetics and breeding, cold / heat shock is usually used, or methods ...

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Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle

In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

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