Method for increasing rearing rate of black carp triploid

A herring and sports technology, applied in fish farming, application, climate change adaptation, etc., can solve the problems of difficult to obtain a four-fold system of purity, difficult to obtain a four-fold system, low success rate, etc., to improve the breeding rate and emergence rate. , Improve the survival rate, reduce the effect of trouble

Inactive Publication Date: 2016-11-23
HUNAN UNIV OF ARTS & SCI
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Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle
In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

Method used

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  • Method for increasing rearing rate of black carp triploid

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Effect test

Embodiment 1

[0017] The method for improving the success rate of triple herring includes the following steps:

[0018] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the tamoxifen content is about 90mg / ml, and wait for use;

[0019] (2) Preparation of tamoxifen injection: Take the above mother solution and directly add it to the prepared oxytocic solution, the ratio is 1ml of tamoxifen mother solution added to 240ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is about 0.375mg / ml, stand-by;

[0020] (3) Injection treatment of the female parent: before the artificial egg laying of the female parent, artificially inject tamoxifen injection into the female parent according to the conventional artificial induction method, and the dosage of tamoxifen injection is about 80 μg / kg;

[0021] (4) Cold shock + hydrostatic press...

Embodiment 2

[0026] The method for improving the success rate of triple herring includes the following steps:

[0027] (1) Tamoxifen inhibitor equipment: Take tamoxifen powder with a purity of more than 95%, add 90% ethanol solution to dissolve, and make a mother liquor, in which the tamoxifen content is about 60mg / ml. use;

[0028] (2) Preparation of tamoxifen injection: Take the above mother solution and directly add it to the prepared oxytocic solution, the ratio is 1ml of tamoxifen mother solution added to 400ml of the prepared oxytocic solution to prepare Injection, the concentration of tamoxifen in the injection is 0.15mg / ml, stand-by;

[0029] (3) Injection treatment of the female parent: before the artificial egg laying of the female parent, the female parent was artificially injected with tamoxifen injection according to the conventional artificial induction method, and the dosage of tamoxifen injection was about 160 μg / kg;

[0030] (4) Cold shock + hydrostatic pressure treatmen...

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Abstract

The invention discloses a method for increasing a rearing rate of black carp triploid. The method comprises the following steps of preparing a tamoxifen inhibitor; preparing tamoxifen injection; treating a female parent by injecting; carrying out cold-shock and hydrostatic pressure treatment; preparing an estrogen inhibitor solution; soaking in the estrogen inhibitor solution at a normal temperature; and obtaining the triploid: taking a male tetraploid as a male parent, taking a common diploid as the female parent, and obtaining a triploid black carp system by adopting a backcross mode. According to the method disclosed by the invention, a 100% black carp triploid pure system can be obtained, the rearing rate and an emergence rate of the triploid are increased, breeding and selecting trouble are reduced; and more importantly, the method is free of pollution on germplasm of natural aquatic varieties and an ecological environment.

Description

technical field [0001] The invention relates to a method for breeding triploid herrings, in particular to a method for improving the triploid success rate of herrings. Background technique [0002] Herring is one of the four major domestic fish species cultured in freshwater in China. The sterility characteristic of triploid herring makes it show more significant advantages in terms of growth speed, stress resistance, disease resistance and meat quality. In the prior art, the sterile triploid herring is obtained by hybridization between tetraploid fish and diploid fish, but to obtain more than 98% triploid fish, then it is necessary to establish genetically stable and extremely high-purity Tetraploid fish. [0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 杨品红刘良国张运生
Owner HUNAN UNIV OF ARTS & SCI
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