A reagent cartridge provided with a magnetic bead transfer structure

Abstract

A reagent cartridge provided with a magnetic bead transferring structure comprises a first cavity and a second cavity provided adjacent to each other, and a separation region separating the first cavity from the second cavity. The separation region is formed with a receiving cavity therein. The receiving cavity is provided with a magnetic bead transferring structure therein for transferring magnetic beads in the first cavity to the second cavity. The magnetic bead transferring structure is sealingly embedded in the receiving cavity, and comprises a rotating column structure and a screw rod driving the rotating column structure to rotate. The screw rod drives the rotating column structure to rotate in the receiving cavity so as to transfer the magnetic beads. By providing the magnetic bead transferring structure in the adjacent cavities, the magnetic beads and a to-be-extracted material attached thereon can be completely and effectively transferred. The reagent cartridge has a simple structure, is convenient to operate, and effectively reduces the complexity of a device cooperating with the reagent cartridge.

Description

technical field [0001] The invention relates to consumables used in the preparation and detection of nucleic acid or protein, in particular to a reagent cartridge provided with a magnetic bead transfer structure. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs fast and accurate detection methods, and molecular detection plays a unique role. The basis of molecular detection is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as formaldehyde-fixed and paraffin-embedded tissues. ) detection can be accurately detected once the disease occurs or even before the symptoms, signs and biochemical changes appear. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip ...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

Therefore, in view of the technical complexity of molecular detection itself, there are few fully automated instrument platforms for "from sample to result" or there are many difficult technical problems. In order to avoid problems in the prior art such as high cost, poor sensitivity, complex structure, overly complicated operation and related detection equipment, etc.

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