SNP primers and screening methods for the screening of pufferfish seedlings

A technology for the screening method of red-finned pufferfish, which is applied in the fields of biochemical equipment and methods, recombinant DNA technology, and the determination/inspection of microorganisms, can solve the problems of labor and material resources, long cycle, low efficiency, etc., and saves labor and material resources. , the effect of reducing the cost of seed selection

Active Publication Date: 2019-10-11
DALIAN OCEAN UNIV
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  • Abstract
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Problems solved by technology

[0003] The traditional breeding method for fish fry is the phenotypic selection method, that is, to select pufferfish with good growth characteristics such as body weight, body length and body length as parents, which requires regular testing and step-by-step screening, which not only consumes a lot of manpower Material resources, long cycle, low efficiency, and the problem that the genetic genes of the selected parents are not good

Method used

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  • SNP primers and screening methods for the screening of pufferfish seedlings
  • SNP primers and screening methods for the screening of pufferfish seedlings
  • SNP primers and screening methods for the screening of pufferfish seedlings

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Experimental program
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Embodiment Construction

[0015] 1. Acquisition of SNP markers related to growth traits of puffer puffer

[0016] 1.1 Acquisition of redfin puffer populations

[0017] The population used is one of the families of red fin puffer hatched by a red fin puffer farm in Dalian. 300 individuals were randomly selected from this family, and the weight, body length and total body length of these 300 individuals were firstly measured. The phenotypic values ​​of growth traits were measured and recorded, and then 100 fish caudal fin rays were cut and stored in 80% ethanol, and stored in a -20°C refrigerator for the extraction of genomic DNA.

[0018] 1.2 Genomic DNA extraction of redfin puffer

[0019] The genomic DNA in the fin rays of puffer puffer was extracted by the conventional phenol imitation method, and the specific steps were as follows:

[0020] (1) Take 100~120 mg of the caudal fin tissue of puffer puffer in a 1.5 ml centrifuge tube, and use ophthalmic scissors to cut it as much as possible (in powde...

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Abstract

The invention discloses a SNP (Single Nucleotide Polymorphism) primer for screening fugu rubripes seedlings and a screening method, which are capable of saving manpower and material resources, lowering seed screening cost and guaranteeing excellent characters of the filial generation. The sequence of the SNP primer is as shown in SEQ ID NO.1 and SEQ ID NO.2. The seedling screening method comprises the following steps: extracting the genomic DNA of to-be-detected fugu rubripes; taking the acquired genomic DNA as a template; performing PCR amplification with upstream primer and downstream primer, thereby obtaining a PCR product; sequencing and genetically typing the obtained PCR product; screening the fugu rubripes with DNA sequence as shown in SEQ ID NO.3 and the No.591 locus gene started from 5' end being homozygosis TT.

Description

technical field [0001] The invention relates to a screening primer and method for puffer puffer seedlings, in particular to a SNP primer and screening method for screening puffer puffer species, which can save manpower and material resources, reduce the cost of seed selection, and ensure good progeny traits method. Background technique [0002] Redfin Pufferfish ( Takifugu rubripes ) is an important economic fish for mariculture in northern my country. [0003] The traditional breeding method for fish fry is the phenotypic selection method, that is, to select pufferfish with good growth characteristics such as body weight, body length and body length as parents, which requires regular testing and step-by-step screening, which not only consumes a lot of manpower Material resources, long cycle, low efficiency, and the problem that the genetic genes of the selected parents are not good. [0004] Molecular marker-assisted breeding is one of the most ideal methods to quickly o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 王秀利于海龙仇雪梅姜志强孟雪松刘圣聪张涛包玉龙
Owner DALIAN OCEAN UNIV
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