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Application of resveratrol in medical titanium alloy implants under condition of diabetes

A technology of resveratrol and diabetes, which is applied in the active ingredients of hydroxyl compounds, medical science, bone diseases, etc., can solve the problems of poor osseointegration, cell dysfunction, apoptosis and other problems of titanium alloy implants, and achieves enhanced osteogenic differentiation. Efficacy, improvement of adhesion state, enhancement of osseointegration effect

Inactive Publication Date: 2016-12-07
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the deficiencies in the prior art, the purpose of the present invention is to provide an application of resveratrol in medical titanium alloy implants under diabetes conditions, to solve the problem of cell dysfunction and Apoptosis, which in turn leads to technical problems of poor osseointegration and loosening of titanium alloy implants

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  • Application of resveratrol in medical titanium alloy implants under condition of diabetes
  • Application of resveratrol in medical titanium alloy implants under condition of diabetes
  • Application of resveratrol in medical titanium alloy implants under condition of diabetes

Examples

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Effect test

Embodiment 1

[0043] This example shows the application of resveratrol in the preparation of drugs for improving the activity of osteoblasts at the "titanium-bone" interface under diabetes conditions, that is, the effect of resveratrol on osteoblasts on the titanium alloy surface in a diabetic environment Improvement of cell viability.

[0044] Experimental method: cutting medical titanium alloy into titanium sheets, grinding and polishing the surface, cleaning and disinfecting for later use. Osteoblasts from the calvaria of SD rats were isolated and cultured, and were divided into 1×10 4 A cell density per milliliter was seeded on the surface of titanium alloy to establish an in vitro "titanium-bone" interface model.

[0045] Divided into 5 groups:

[0046] 1) normal serum group (normal group);

[0047] 2) diabetes serogroup (diabetes group);

[0048] 3) Diabetes + resveratrol 10μM;

[0049] 4) Diabetes + resveratrol 50μM;

[0050] 5) Diabetes + resveratrol 250 μM.

[0051] The amou...

Embodiment 2

[0055] This example shows the application of resveratrol in the preparation of drugs that enhance the osteogenic differentiation of osteoblasts at the "titanium-bone" interface under diabetes conditions, that is, the effect of resveratrol on osteoblasts on the surface of titanium alloys in a diabetic environment is given. enhancement of osteogenic differentiation.

[0056] Experimental method: divide osteoblasts into 1×10 4 Cells per milliliter were seeded on the surface of titanium alloy for culture, and were divided into 5 groups:

[0057] 1) Normal group;

[0058] 2) Diabetes group;

[0059] 3) Diabetes + resveratrol 10μM;

[0060] 4) Diabetes + resveratrol 50μM;

[0061] 5) Diabetes + resveratrol 250 μM.

[0062] After 7 days of culture, use the ALP enzyme activity detection kit (Sigma company) to detect the degree of osteoblast differentiation on the titanium surface (ALP activity reflects osteogenic differentiation), and operate according to the professional instruc...

Embodiment 3

[0066] This example shows the application of resveratrol in the preparation of drugs to improve the adhesion state of osteoblasts at the "titanium-bone" interface on the material surface under diabetes conditions, that is, the effect of resveratrol on osteoblasts in diabetes environment is given. Improvement of adhesion state and cell proliferation on titanium alloy surface.

[0067] Experimental method: Osteoblasts were inoculated on the surface of titanium alloy for culture, and divided into 5 groups:

[0068] 1) Normal group;

[0069] 2) Diabetes group;

[0070] 3) Diabetes + resveratrol 10μM;

[0071] 4) Diabetes + resveratrol 50μM;

[0072] 5) Diabetes + resveratrol 250 μM.

[0073] The cytoskeleton and nucleus of the osteoblasts were stained with fluorescence after 3 days of culture, and then observed and photographed with a laser confocal microscope. Each group collected pictures on three titanium sheets (the experiment was repeated 3 times), and 6 randomly selecte...

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Abstract

The invention provides application of resveratrol in medical titanium alloy implants under the condition of diabetes. The application has the advantages that by establishing a medical titanium alloy surface osteogenesis cell culture system, the following key treatment functions of the resveratrol are demonstrated, namely that the cell activity of titanium-bone interface osteogenesis cell in diabetes is improved; the osteogenic differentiation efficiency of the osteogenesis cell is improved; the adhesion state of the osteogenesis cell on the material surface is improved; the capacity of the osteogenesis cell resisting oxidizing stress injury caused by diabetes environment is improved; the pathological apoptosis of the osteogenesis cell is reduced; in addition, the osseointegration effect of the medical titanium alloy implant in a diabetes animal is improved by the resveratrol; the resveratrol can be used as a new drug or titanium alloy implant functional improving composite material ingredient to improve the osseointegration effect of the titanium alloy implant of a diabetes patient.

Description

technical field [0001] The invention belongs to the field of medical biomaterials, and relates to the osseointegration effect of medical titanium alloy implants, in particular to the application of resveratrol in medical titanium alloy implants under diabetes conditions. Background technique [0002] The loosening of titanium alloy implants caused by diabetes is a medical problem that needs to be solved urgently: titanium alloy is the most widely used bone implant material, but in some specific disease groups such as diabetic patients, the instability rate of titanium alloy implants is significantly increased , seriously affect the prognosis, and cause a great burden on the family and society of the patient. Therefore, it is a medical problem to be solved urgently to clarify the mechanism of the instability of titanium alloy implants and take effective measures to reduce the instability rate of titanium implants. [0003] Cell dysfunction and apoptosis caused by oxidative s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P19/08A61L27/06A61L27/54
CPCA61K31/05A61L27/06A61L27/54A61L2300/216
Inventor 胡晓帆冯亚非雷伟王林陆奕兆相庚
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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