Protection solution for preparing decellularized corneas

Abstract

The invention provides a protection solution for preparing decellularized corneas. The protection solution is a PBS or HBSS buffer solution, wherein 5-12 g / L of hyaluronic acid, 5-20 g / L of chondroitin sulfate, and 3-10 g / L of low molecular weight dextran are added in the PBS or HBSS buffer solution. According to the protection solution, protective ingredients are added in the basic buffer solution, the prepared protection solution can maintain the colloid osmotic pressure of a preparing solution of decellularized corneas through selection of components and a proportion, and compared with a traditional preparing solution for decellularized corneas, cornea transparency decreasing caused by excessive swelling and missing of extracellular matrix ingredients such as collagen in the decellularization treatment process in a later stage can be remarkably relieved; besides, antibiotic ingredients such as tobramycin are added, breeding of bacteria such as pseudomonas aeruginosa, klebsiella biofilm, enterobacter and proteus which are likely to infect corneas is suppressed, and the contamination probability in the treatment process is avoided.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a protective solution for preparing acellular cornea. Background technique [0002] Corneal disease is the second leading cause of blindness in my country. There are about 4 million corneal blind patients in China, and about 100,000 new corneal blind patients are added every year. However, only about 5,000-8,000 corneal transplants can be performed each year in China. The serious shortage of corneal donors leads to the vast majority of patients waiting in line in the dark, and some even miss the best opportunity for treatment, resulting in permanent blindness. Corneal transplantation is divided into penetrating keratoplasty, lamellar keratoplasty and corneal endothelial transplantation. Among them, lamellar keratoplasty has lower requirements for donor materials, and is especially suitable for patients with no obvious lesions of corneal limbal stem cells an...

AI Technical Summary

Problems solved by technology

Swelling for a long time will often lead to the destruction of the corneal collagen structure, accompanied by the loss of corneal transparency, poor optical effect, gradually thinning or long-term opacity after transplantation, and the goal of long-term vision recovery cannot be achieved