Method for quick screening and assessment of pesticide residues in tea

A technology for pesticide residues and tea, which is applied in the biosafety assessment and application field of carbamate pesticide residues in tea, and can solve the problems affecting the accuracy and precision, sensitivity and specificity of microorganisms, and the lack of screening for butyric acid bacteria Work strains and other problems, to achieve the detection method is simple and easy, high sensitivity, good specificity effect

Active Publication Date: 2016-12-14
WENZHOU MEDICAL UNIV
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AI Technical Summary

Problems solved by technology

However, the sensitivity and specificity of the working strain to the substance to be tested is the most critical issue affecting the accuracy and precision of the microbiological method
So far, there is no report on the screening of butyric acid working strains with high sensitivity and specificity for carbamate pesticides and its application in the screening of carbamate pesticide residues in tea

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The screening of embodiment 1 sensitive strain

[0030] Isolation and identification of butyric acid bacteria WZ001, straight or curved bacillus, 0.6-1.2×3.0-7.0μm, round end, single, paired, short chain, and occasionally long filamentous bacteria. Movement with perinatal flagella, spores ovoid, eccentric to subterminal, without exine and appendage filaments. Gram-positive, becoming negative in older cultures; often amyloid-positive. The cell wall contains DH-diaminopimelic acid; glucose is the only cell wall sugar. Light microscopy findings were consistent with the morphology of Clostridium butyricum. According to the "Bergery Bacterial Identification Manual", it can be judged that the bacteria belong to Clostridium butyricum. Some sugars can be fermented to produce hydrogen and butyric acid. Visible colonies in the solid medium are milky white, round and slightly convex, with irregular edges, 1-3 mm in diameter, slightly shiny surface, and bubbles in the liquid me...

Embodiment 2

[0031] Example 2 Screening of Tea Methomyl Residues

[0032] (1) Screening medium preparation:

[0033] Peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, K 2 HPO 4 2g, MgSO 4 ·7H2 O0.5g, MnSO 4 4H 2 O 0.2g, fructooligosaccharide 3g, diammonium citrate 2g, Tween-80 1mL, agar 15g, distilled water 1L, heat and dissolve the above ingredients to correct pH 6.5, autoclave at 115°C for 15-20 minutes;

[0034] (2) Bacterial suspension preparation:

[0035] Butyric acid bacteria WZ001 was inoculated on the slant medium of nutrient agar, cultured anaerobically at 35±2°C for 24 hours, washed the bacterial lawn with sterilized physiological saline, made a suspension, and transmitted light at a wavelength of 580nm in a 721 spectrophotometer. The excess rate is controlled at 0.7, and stored in a refrigerator at 2-8°C;

[0036] (3) Bacteria culture medium preparation:

[0037] Take a plate with a diameter of about 90mm and a height of 16-17mm, inject 20...

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Abstract

The invention provides a method for quick screening and assessment of pesticide residues in tea. The invention provides a microbial method for screening carbamate pesticide residues in tea by using clostridum butyricum WZ001 as a working strain. The size of inhibition zone on a screening medium is employed to determine whether the acephate residues in a food sample to be measured exceeds the standards. The method for quick screening and assessment of pesticide residues in tea has the characteristics of simple operation, low cost, high sensitivity, and suitability for screening sample characteristics, is superior to the current national standards for rapid screening of pesticide residues, fully meets the requirements for maximum residue limit in food of national food safety standards, has good ability of matrix interference resistance, and can effectively avoid false positive results.

Description

technical field [0001] The invention relates to the detection of pesticide residues in tea, in particular to the biological safety assessment and application of carbamate pesticide residues in tea. Background technique [0002] Pesticide residues in tea are a food safety issue that the public is very concerned about. The current detection of pesticide residues in my country generally uses instrumental analysis methods, mainly using large-scale instruments such as gas chromatography, liquid chromatography, and gas-liquid chromatography-mass spectrometry. The instruments are expensive and difficult to operate. Personnel must have considerable professional skills, and the detection cycle is long, which is not conducive to popularization in front-line supervision departments. The instrumental analysis method can only detect a single pesticide separately, and cannot fully reflect the biological safety of food. Moreover, the detection time is long, the equipment is expensive, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12R1/01
CPCC12Q1/04C12N1/205C12R2001/01
Inventor 刘佳明孙晶
Owner WENZHOU MEDICAL UNIV
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