Urine sulfhydryl compound detection reagent and preparation method thereof

A sulfhydryl compound and detection reagent technology, which can be applied in material analysis by observing the effect on chemical indicators, measuring devices, and analysis by chemical reaction of materials, etc. The effect of detection accuracy

Inactive Publication Date: 2016-12-14
SUZHOU RELING BIOTECH CO LTD
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Problems solved by technology

[0002] The current clinical cervical cancer screening and auxiliary examinations include the following: 1. Pap smear cytology examination 2. Iodine test 3. Cervix and cervical canal biopsy 4. Colposcopy Colposcopy cannot directly diagnose cancer 5 , Cervical conization 6, cervical photography 7, fluoroscopy 8, tumor biochemical diagnosis, the above examination methods are all invasive, it is necessary to provide non-invasive and highly accurate detection reagents

Method used

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Embodiment Construction

[0014] The present invention will be further described in detail below in conjunction with specific embodiments.

[0015] A preparation method of urine mercapto compound detection reagent, is characterized in that, comprises the steps:

[0016] (1) Prepare a mixed solution of mercury dichloride, magnesium chloride and sodium acetate;

[0017] (2) Prepare a mixed solution of phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid, the pH of the mixed solution is 4.5;

[0018] (3) When used, the volume ratio of the solution prepared in step (1) to the solution prepared in step (2) is 2:1;

[0019] (4) The control solution is distilled water.

[0020] The concentration of mercury dichloride in the mixed solution in step (1) is 0.01mol / L, the concentration of magnesium chloride is 0.005mol / L, and the mass concentration of sodium acetate is 9wt%. In step (2), the concentration of phosphotungstic acid is 52g / L, the concentration of sodium dihydrogen phosphate is 9wt%,...

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Abstract

The invention relates to the field of detection reagents, and in particular relates to a urine sulfhydryl compound detection reagent and a preparation method thereof. The preparation method comprises the following steps: preparing mixed solution of mercury bichloride, magnesium chloride and sodium acetate; preparing mixed solution of phosphotungstic acid, sodium dihydrogen phosphate and sulphuric acid, wherein pH of the mixed solution is 3-5. The detection reagent comprises first mixed solution of bichloride, magnesium chloride and sodium acetate and second mixed solution of phosphotungstic acid, sodium dihydrogen phosphate and sulphuric acid, wherein pH of the second mixed solution is 3-5; when the detection reagent is used, volume ratio of the first mixed solution to the second mixed solution is (1-2):1, and reference solution is distilled water. The detection reagent provided by the invention is convenient in sampling, simple in operation and rapid in report, can detect cervical carcinogenesis at an earlier period and is a preferable detection method.

Description

technical field [0001] The invention relates to the field of detection reagents, in particular to a urine mercapto compound detection reagent and a preparation method. Background technique [0002] The current clinical cervical cancer screening and auxiliary examinations include the following: 1. Pap smear cytology examination 2. Iodine test 3. Cervix and cervical canal biopsy 4. Colposcopy Colposcopy cannot directly diagnose cancer 5 , Cervical conization 6, cervical photography 7, fluoroscopy 8, tumor biochemical diagnosis, the above inspection methods are all invasive, it is necessary to provide non-invasive and high accuracy detection reagents. Contents of the invention [0003] Aiming at the deficiencies of the prior art, the invention provides a urine mercapto compound detection reagent with high sensitivity, simple operation and high accuracy and a preparation method. [0004] In order to achieve the above-mentioned purpose of the invention, the present invention a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 扈晓佳梅宗泉胡梦丽
Owner SUZHOU RELING BIOTECH CO LTD
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