Preservative for fetal cell-free dna in peripheral blood of pregnant women and vacuum blood collection tubes composed of it

A technology for maternal peripheral blood and preservatives, applied in the field of vacuum blood collection tubes, can solve the problems of short storage time and easy degradation of fetal cell-free DNA, and achieve the advantages of preventing the release of cell chromosomal DNA, inhibiting DNase-mediated degradation, and improving stability. Effect

Active Publication Date: 2019-09-20
CHENGDU RICH SCI IND
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  • Abstract
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Problems solved by technology

[0005] The technical problem to be solved by the present invention is: there is no collection and storage technology for the in vitro detection of free DNA in the peripheral blood of mature pregnant women, which leads to the short storage time and easy degradation of fetal free DNA in the existing plasma. A preservative for fetal cell-free DNA in the peripheral blood of pregnant women and the vacuum blood collection tube it consists of

Method used

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  • Preservative for fetal cell-free dna in peripheral blood of pregnant women and vacuum blood collection tubes composed of it
  • Preservative for fetal cell-free dna in peripheral blood of pregnant women and vacuum blood collection tubes composed of it
  • Preservative for fetal cell-free dna in peripheral blood of pregnant women and vacuum blood collection tubes composed of it

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Embodiment 1

[0026] A preservation agent for fetal free DNA in the peripheral blood of pregnant women, including cell stabilizers, anticoagulants, buffers, metabolic inhibitors and DNase inhibitors.

[0027] In the present embodiment, the cell stabilizer is diazolidinyl urea and NaF, the anticoagulant is EDTA potassium salt, the buffer is a standard Tris-Cl buffer with a final concentration of 10 mM, and the metabolic inhibitor is molecular biological Grade glycogen, the DNase inhibitor is dithiothreitol (DTT).

[0028] Wherein, the proportioning ratio of each component is specifically set as shown in Table 1.

[0029] Table 1

[0030]

Embodiment 2

[0032] The difference between this embodiment and embodiment 1 is that in this embodiment, NaF in embodiment 1 is replaced by formaldehyde with a concentration of 40% as example 4-6, and the specific settings are as follows:

example 4

[0033] Example 4: The NaF in Example 1 was replaced by 40% formaldehyde in an amount of 30 μl;

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Abstract

A preservative for free fetal DNA in peripheral blood of a pregnant woman and a vacuum blood collection tube comprising the same, for solving the problems of a short preservation time and easy degradation of free fetal DNA in existing plasma due to currently immature collection and preservation technologies for in vitro detection of free DNA in peripheral blood of a pregnant woman. The preservative comprises a cell stabilizer, an anticoagulant, a buffer, a metabolic inhibitor, and a DNase inhibitor, and can stabilize nuclear cells in maternal peripheral blood, prevent the release of cell chromosomal DNA, and inhibit DNase-mediated degradation, contributing to the overall stability of free fetal DNA.

Description

technical field [0001] The invention relates to the field of prenatal diagnosis, in particular to a preservative for fetal free DNA in the peripheral blood of pregnant women and a vacuum blood collection tube composed of the same. Background technique [0002] Non-invasive prenatal diagnosis is mainly based on the free fetal DNA in the plasma of pregnant women. Pregnant women can detect fetal free DNA from the 7th week of pregnancy. The use of fetal free DNA can be used for early and specific prenatal screening and diagnosis. [0003] However, the amount of fetal free DNA in the plasma of pregnant women is very small, accounting for only about 5% to 7% of the total free DNA, and it is extremely vulnerable to chromosomal DNA pollution released by the rupture of maternal nucleated cells and high concentration of intracellular DNase degradation . [0004] Studies have shown that pre-analytical factors for cell-free fetal DNA testing, such as improper blood sample collection, s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/078C12M1/24
Inventor 韦德
Owner CHENGDU RICH SCI IND
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