PCR technology-based quail sex identification method

Abstract

The invention discloses a PCR technology-based quail sex identification method, and belongs to the technical field of biological detection identification. Two pairs of PCR primers used for PCR identification comprise a pair of micro-satellite primers and a pair of internal control gene (beta-actin) primers. The PCR identification method comprises the following steps: carrying out PCR amplification by adopting the quail sex identification PCR primers with the blood sample genome DNA of a young quail to be detected as a template, detecting the above obtained PCR amplification product by adopting agarose gel electrophoresis, judging the young quail to be detected as a male quail when the amplification product only has a 259 bp specific amplification band, and judging the young quail to be detected as a female quail when the amplification product has a 259 bp specific amplification band and a 114 bp specific amplification band. The method has the advantages of simplicity and convenience in operation, realization of sex discrimination in at the hatching time, rapidness in discrimination, accurate result, and avoiding of the disadvantages of long time, high breeding cost and high error rate of traditional methods.

Description

technical field [0001] The invention belongs to the technical field of biological detection and identification, and in particular relates to a method for quail sex identification based on PCR technology, which can be applied to early sex identification of baby quails. Background technique [0002] Quail is a special economic poultry and the smallest poultry breed, which has extremely high edible value and therapeutic effect. Quail eggs are rich in nutrition, high in protein and low in cholesterol. It can be seen that the breeding of quail for eggs has a good market prospect. Since the weight of quail eggs hatched is only about 7 grams, usually commercial egg substitutes use quails to distinguish males and females by feather color; and parental quails have the same coat color, so males and females cannot be distinguished when hatching, and generally have to wait for the quails to mature. It is not until about 30 days old that males and females can be distinguished by body sh...

AI Technical Summary

Problems solved by technology

The advantage of this method is that the cost is low, and it is widely used under the existing identification conditions. The disadvantage is that the operation is cumbersome, the time is long, and it is easy to cause pollution.

In recent years, some scholars have redesigned primers to distinguish ZW bands by using the different melting temperatures of CHD-W and CHD-Z amplification products, but the amplification reactions of CHD-Z and CHD-W were carried out in two PCR reaction tubes respectively. This not only increases the economic cost, but also has low practicability, and on the other hand, misjudgment may occur; another report shows that CHD-W and CHD-Z are amplified with the same primers in the same tube, and the amplified fragments are used to Z and W have 3 SNPs, and the melting temperature of their amplified products is different to distinguish. This method only uses a pair of primers, and the sequence and size of the generated fragments are very close, so they cannot be distinguished by gel electrophoresis; the disadvantages are: Users who do not have a melting curve analyzer cannot use it, and lack a simple and effective method to verify the results

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