System for processing DNA sequencing images pre-grouped according to similarity

Abstract

The invention relates to a system for processing DNA sequencing images pre-grouped according to similarity. The system comprises a reaction chip, a CCD camera and a CCD camera acquisition module, wherein a to-be-sequenced liquid passes through the reaction chip and reacts with a reaction liquid in the reaction chip; the CCD camera is arranged on one side of a DNA sequencing instrument and used for acquiring images of reaction information on a sequencing chip; the CCD camera acquisition module is connected with the CCD camera and acquires shot picture information; and the CCD camera acquisition module acquires image information stored in the CCD camera according to a preset program. According to the system, when the image information is processed, the image information is not compared with standard basic group images according to an image acquisition sequence, images of similar information are grouped and intensively compared in advance, and finally basic groups are re-sorted through a time sequence, so that basic group information is finally output.

Description

technical field [0001] The invention relates to the field of DNA sequencing analysis, in particular to a DNA sequencing image processing system pre-grouped according to similarity. Background technique [0002] In the field of DNA sequencing technology, pyrosequencing technology (pyrosequencing) is a new enzyme cascade sequencing technology developed by Nyren et al. in 1987. Its repeatability and accuracy are comparable to Sanger method DNA sequencing technology. , while the speed is greatly improved. [0003] The overall operation process is described as follows: After the DNA sample is crushed, the library construction reagent is used to add adapters, single-strand capture, bind to microspheres, microemulsion PCR amplification, and emulsion breaking to obtain a DNA library built on microspheres. The template lays the library and the enzymes required for the sequencing reaction on the sequencing chip with a micro-reaction pool, installs the sequencing chip and sequencing r...

AI Technical Summary

Problems solved by technology

[0004] In the actual DNA sequencing process, the sequencer is required to have continuous and controllable characteristics in the reagent supply and sequencing reaction process, and the reaction process and results of the final sequencing chip are captured by a CCD camera, and the control of the CCD camera The system determines the type of bases, A, T, C, G, through the collected continuous images, thereby determining the reddest DNA sequence. However, when the control system is analyzing, it often only intercepts and analyzes the current fragment, which is difficult to analyze. Intermittent fragments are compared and judged, so the accuracy is not high

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