A method for removing broad bean wilt virus No. 2 from pepper seeds

A technology for seeds and peppers, applied in the field of removing broad bean wilt virus No. 2 in pepper seeds, can solve the problem of difficulty in determining the quantity and activity of seed viruses, inability to accurately control the technical indicators of passivation viruses, and no detoxification effect of pepper seeds has been seen. Evaluation and other issues, to achieve the effect of fast detection efficiency, shortening detection time, and improving germination potential

Active Publication Date: 2019-12-06
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the commonly used detection methods for the detoxification effect of seeds mainly include observation of symptoms at seedling stage, indicator plant method, ELISA, immunoelectron microscopy and RT-PCR technology. Although these methods can evaluate the detoxification effect, there are some stability Due to the disadvantages of poor sex, only qualitative detection, and limited sensitivity, it is impossible to accurately quantify the virus, and it is difficult to determine the impact of seed detoxification treatment measures on the number and activity of the virus, so it is impossible to accurately control the technical indicators of the inactivated virus, and it is difficult to avoid virus removal. The effect is not good, or the virus in the seeds is removed, but the quality of the seeds is seriously reduced
At present, there is no report on the evaluation of detoxification effect of pepper seeds by using TaqMan probe real-time fluorescent PCR technology

Method used

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  • A method for removing broad bean wilt virus No. 2 from pepper seeds
  • A method for removing broad bean wilt virus No. 2 from pepper seeds
  • A method for removing broad bean wilt virus No. 2 from pepper seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Comparison of detoxification effects of several detoxification methods

[0037] In this embodiment, seven detoxification methods are designed to detoxify pepper seeds, with non-detoxified pepper seeds as a control, and the amount of seeds for each treatment is about 300 full and poisonous pepper seeds. After the seeds were detoxified, the TaqMan probe real-time fluorescent quantitative PCR detection method was used to quantitatively detect BBWV2 in pepper seeds, and the detoxification effects of various detoxification methods were compared.

[0038] 1. The detoxification method is designed as follows:

[0039] (1) Dry heat treatment method: Place the pepper seeds to be detoxified in an oven, heat-air for 48 hours at 70 ℃, and then stand to cool.

[0040] (2) Humidity heat treatment method: soak the pepper seeds to be detoxified in 5 times the volume of boiling water, and quickly shake to cool to 40-45 ℃, drain the aqueous solution, and put the treated seeds on the ne...

Embodiment 2

[0063] Example 2. The effect of several detoxification methods on the germination rate and germination potential of pepper seeds

[0064] Randomly select 150 capsicum seeds of each detoxification treatment and non-detoxification treatment in Example 1, and divide them into 3 groups. After soaking in cold water for 4 hours, use a petri dish filter paper constant temperature germination method to seed in a 28℃ constant temperature incubator For the germination test, from the 3rd day after the treatment, the germination situation was observed and counted daily, and the germination number was recorded. The germination potential was determined by the germination number of each detoxification treatment on the 7th day, and the germination test was ended on the 10th day. Germination rate (%) = (number of germinated seeds / number of tested seeds)×100; germination potential (%)=(number of seeds germinated within the specified time / number of tested seeds)×100.

[0065] The results of the seed ...

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Abstract

The invention relates to a method for removing No.2 broad bean wilt virus in chili seeds and belongs to the technical field of treatment. Specifically, the method comprises the following steps: (1) carrying out moisture-thermal treatment; (2) carrying out pesticide treatment; (3) filtering seeds; (4) poaching; (5) drying; (6) detecting viruses. According to the method provided by the invention, the disadvantages of the prior art are overcome; when the method provided by the invention is utilized, the germination rate and germinating potential of the chili seeds are improved, and the removal of the No.2 broad bean wilt virus in the chili seeds is more complete; the removal rate reaches 100 percent. Furthermore, the invention provides a TaqMan probe real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method capable of evaluating a virus removing effect; the content of the viruses in the virus-removed seeds can be accurately grasped, so that technical indexes of passivated viruses are accurately controlled under the condition that high quality of the seeds is guaranteed, and the virus activity is inhibited to the greatest extent. The method for removing the viruses of the chili seeds, provided by the invention, is not limited by time and influenced by environment, and can be used in a laboratory or a basic unit all year round; the method is very practical and can be used for large-scale production of the virus-removed chili seeds.

Description

Technical field [0001] The invention relates to the technical field of seed treatment, in particular to a method for removing broad bean wilt virus No. 2 in pepper seeds. Background technique [0002] Pepper is the second largest vegetable crop in my country, with an annual cultivation area of ​​about 1.33 million ha 2 . Virus disease is an important disease that endangers pepper production, causing a 20%-70% reduction in pepper production and deteriorating quality, which has become an important obstacle restricting the development of pepper industry. Broad bean wilt virus (BBWV) is a worldwide epidemic virus with an extremely wide range of hosts, which can infect 328 species of plants in 44 families and 186 genera. According to different serotypes, broad bean wilt virus is divided into two species, namely BBWV1 and BBWV2. The isolates obtained in my country are mainly BBWV2, and BBWV1 has not been found yet. The virus was first discovered in Australia. Since the first report i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/08C12Q1/70C12Q1/68
CPCA01C1/00C12Q1/686C12Q1/6895C12Q1/701C12Q2561/113C12Q2563/107C12Q2545/114C12Q2561/101C12Q2531/113
Inventor 罗明韩剑张强赵冰梅张祥林徐金虹
Owner XINJIANG AGRI UNIV
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