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Bromelain production method

A technology of bromelain and a production method, which is applied in the production field of bromelain, can solve the problems of easily damaged concentrating membrane, high pressure of booster pump, shortening the service life of concentrating membrane, etc., achieves high interception, reduces production load, and reduces microfiltration effect of stress

Inactive Publication Date: 2017-01-04
HEFEI XINDA MEMBRANE TECH
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Problems solved by technology

[0003] At present, the main production methods of bromelain include physical extraction methods and chemical production methods. With the development of membrane technology, concentrated membrane separation and extraction of bromelain has been widely used, but in the separation process, because pineapple juice contains more impurities, so The pressure of the booster pump is high, and the concentration membrane is easily damaged, which reduces the service life of the concentration membrane

Method used

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  • Bromelain production method

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Embodiment

[0023] Such as figure 1 As shown, a kind of bromelain production method of the present invention comprises the following steps: cleaning treatment of pineapple raw material, crushing, squeezing, two-stage centrifugation, ceramic membrane microfiltration, concentrated ultrafiltration, and drying to obtain a finished product.

[0024] Specifically: peel the pineapple raw material, put it into the cleaning tank and clean it, then use a pulverizer to pulverize the pineapple at a stirring speed of 200r / min; the pulverized pineapple is transported to the juicer by a conveyor belt for squeezing to obtain pineapple Suspension: Put the pineapple suspension into a centrifuge and centrifuge at a speed of 220 r / min for 30 minutes to obtain the clarified pineapple juice of the first centrifugation, and then put the clarified pineapple juice into the centrifuge again at the same speed and time after standing for 5 minutes Perform secondary centrifugation to obtain the final clarified pineap...

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Abstract

The invention discloses a bromelain production method which includes the steps: firstly, cleaning; secondly, crushing; thirdly, squeezing juice; fourthly, performing two-stage centrifugation; fifthly, performing ceramic membrane microfiltration; sixthly, performing ultrafiltration and concentration; seventhly, drying. According to the bromelain production method, two-stage centrifugation of the squeezed pineapple juice is achieved, purity of clarification pineapple juice is improved, microfiltration pressure is reduced, ceramic membrane microfiltration is performed, high rejection of living bacteria under the physical state is achieved, downstream enzyme clear solution with high clarity is separated, production load of a downstream concentration process is reduced, part of pigment and impure protein are removed by the aid of ultrafiltration and concentration, quality and stability of products are improved, ultrafiltration and concentration are performed at normal temperature, enzyme activity is not lost, and bromelain yield is improved.

Description

technical field [0001] The invention designs a method for producing medical mold, in particular relates to a method for producing bromelain. Background technique [0002] Bromelain is a natural plant protease extracted from pineapple fruit, stems, and leaves, which is refined, purified, concentrated, and freeze-dried. It has a strong ability to decompose fibrin and blood clots, and at the same time It can also promote drug absorption and inhibit the growth of tumor cells. [0003] At present, the main production methods of bromelain include physical extraction methods and chemical production methods. With the development of membrane technology, concentrated membrane separation and extraction of bromelain has been widely used, but in the separation process, because pineapple juice contains more impurities, so The pressure of the booster pump is high, and the concentration membrane is easily damaged, which reduces the service life of the concentration membrane. Contents o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50
CPCC12N9/63C12Y304/22004
Inventor 陈传云梁远争李胜明
Owner HEFEI XINDA MEMBRANE TECH
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