Composite product of ganoderma lucidum polysaccharides and egg yolk antibody
A technology of egg yolk antibody and Ganoderma lucidum polysaccharide, which is applied in the direction of antibodies, medical preparations containing active ingredients, antiviral agents, etc., to achieve the effect of large market promotion value, low cost, and enhanced effect
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Embodiment 1
[0022] Embodiment 1: A preparation method of a compound product of Ganoderma lucidum polysaccharide and egg yolk antibody, the steps are as follows:
[0023] (1) Preparation of Ganoderma lucidum polysaccharide liquid: prepare Ganoderma lucidum solid fermentation medium, pack it into bags, autoclave at 116°C for 1.2 hours, inoculate the strains of Ganoderma lucidum grains, cultivate at 25°C until the bottom of the bag is covered with bacteria, and place in hot water at 100°C Extract for 0.5 hours, centrifuge, and take the supernatant;
[0024] (2) Preparation of egg yolk antibody: According to the conventional method, prepare high-immunity eggs against chicken Newcastle disease virus, sterilize, break the eggs, separate the egg yolk, add 6 times the volume of purified water, homogenize in a tissue homogenizer, and add a volume ratio of 8.5% octanoic acid, let stand overnight at 4°C, centrifuge at 3000r for 15 minutes, remove the supernatant, and sterilize with formaldehyde;
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Embodiment 2
[0026] Embodiment 2: A preparation method of a compound product of Ganoderma lucidum polysaccharide and egg yolk antibody, the steps are as follows:
[0027] (1) Preparation of Ganoderma lucidum polysaccharide liquid: prepare Ganoderma lucidum solid fermentation medium, pack it into bags, autoclave at 126°C for 2.8 hours, inoculate the strains of Ganoderma lucidum grains, culture at 25°C until the bottom of the bag is covered with bacteria, and place in hot water at 100°C Extract for 1.5 hours, centrifuge, and take the supernatant;
[0028] (2) Preparation of egg yolk antibody: According to the conventional method, prepare high-immune eggs against chicken Newcastle disease virus, sterilize, break the eggs, separate the egg yolk, add 14 times the volume of purified water, and homogenize in a tissue homogenizer, adding a volume ratio of 11.5% octanoic acid, let stand overnight at 4°C, centrifuge at 3000r for 45 minutes, remove the supernatant, and sterilize with formaldehyde;
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Embodiment 3
[0030] Embodiment 3: A preparation method of a compound product of Ganoderma lucidum polysaccharide and egg yolk antibody, the steps are as follows:
[0031] (1) Preparation of Ganoderma lucidum polysaccharide liquid: prepare Ganoderma lucidum solid fermentation medium, pack it into bags, autoclave at 119°C for 1.6 hours, inoculate the strains of Ganoderma lucidum grains, cultivate at 25°C until the bottom of the bag is covered with bacteria, and place in hot water at 100°C Extract for 0.7 hours, centrifuge, and take the supernatant;
[0032] (2) Preparation of egg yolk antibody: According to the conventional method, prepare high-immunity eggs against chicken Newcastle disease virus, sterilize, break the eggs, separate the egg yolk, add 8 times the volume of purified water, homogenize in a tissue homogenizer, and add a volume ratio of 9.5% octanoic acid, let stand overnight at 4°C, centrifuge at 3000r for 25 minutes, remove the supernatant, and sterilize with formaldehyde;
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