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Reproduction and regeneration method applied to genetic integrity analysis of Glycine soja Sieb.et Zucc.

A technology of wild soybeans and updated methods, applied in the direction of botany equipment and methods, applications, organic fertilizers, etc., can solve the problems of irregular emergence, long dormant period, low germination rate, etc., and achieve the promotion of germination and rooting, high reliability and Accuracy, the effect of improving seedling raising effect

Active Publication Date: 2017-02-01
SHANDONG CROP GERMPLASM CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, wild soybean seeds have hardening phenomenon, long dormant period under natural conditions, low germination rate, and irregular emergence

Method used

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  • Reproduction and regeneration method applied to genetic integrity analysis of Glycine soja Sieb.et Zucc.
  • Reproduction and regeneration method applied to genetic integrity analysis of Glycine soja Sieb.et Zucc.
  • Reproduction and regeneration method applied to genetic integrity analysis of Glycine soja Sieb.et Zucc.

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 Apply the method of the present invention and the conventional method to carry out breeding renewal and genetic integrity analysis to wild soybean

[0054] (1) Test materials: wild soybean seeds from Laoshan Mountain were used as test materials.

[0055] (2) Test method:

[0056] 1. Method one: use the method provided by the invention to reproduce and update Laoshan wild soybeans. Proceed as follows:

[0057] (1) Reproduction and reproduction of Laoshan wild soybean parental treatment

[0058] ① Breeding site selection:

[0059] a. Breeding area: select the germplasm origin. Since the origin of wild soybeans in Laoshan is Laoshan District, Qingdao City, Shandong Province, a local farmer was found and the land was leased for reproduction and renewal, which can satisfy the growth and development of the reproduction and renewal materials and the expression of their traits.

[0060] b. Experimental site: flat terrain, uniform soil strength, regular shape, ...

Embodiment 2

[0149] Embodiment Two Wild Soybean Breaks Hard Solid Method Comparison

[0150] (1) Test materials and methods

[0151] 1. Test material

[0152] The test materials are mature annual wild soybean seeds, which were collected from Laoshan District, Qingdao City in 2014, that is, Laoshan wild soybean.

[0153] 2. Method

[0154] (1) Determination of seed moisture and thousand-grain weight

[0155] Refer to GB / T3543.4 for the inspection of crop seeds.

[0156] (2) Determination of seed hardness rate

[0157] Randomly pick 100 × 2 wild soybean seeds and soak them in water at room temperature for 24 hours, count the number of non-imbibed seeds, and calculate the firmness rate.

[0158] (3) The method of breaking the hard solid

[0159] 1. The method of the present invention: according to the following proportions: 6~7mg / L of thalassin and 20~30mg / L of lemongrass water extract are prepared into a solution with warm water at 35~50°C. Put wild soybeans into the solution for soak...

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Abstract

The invention provides a reproduction and regeneration method applied to genetic integrity analysis of Glycine soja Sieb.et Zucc. The method comprises the following steps: performing artificial aging treatment on parental seeds, and selecting parent processes of different germination rate gradients; breaking hard seeds, growing seedlings, and transplanting the seedlings into a field; harvesting individual plants; treating filial generations, and performing seedling growing, transplanting, morphological character investigation and individual plant harvesting according to the same steps of the parent processes. Filial generation individual plants correspond to parental generations one by one, namely, the numbers of individual plants in processes of the filial generations correspond to parental individual plants one by one. In the reproduction and regeneration period of the parental generations and the filial generations, morphological marker analysis and SSR (Simple Sequence Repeat) molecular marker analysis are performed to detect the genetic integrity. A method for breaking hard seeds of the Glycine soja Sieb.et Zucc.. is innovated. The method is complete, feasible, systematic and standardized; the method can be applied to reproduction and regeneration of stocked Glycine soja Sieb.et Zucc.. with high activity in order to better maintain the genetic integrity.

Description

technical field [0001] The invention belongs to the field of crop germplasm resources, and relates to a breeding and updating method for wild soybean genetic integrity analysis. Background technique [0002] Wild soybean (Glycine soja Sieb.et Zucc.) is the direct ancestor of cultivated soybean. Wild soybean grown under natural conditions has never been affected by artificial selection in genetics, so many rich functional genes have not been lost due to artificial selection. , these genes are likely to be the key genes that are urgently needed in soybean production. Wild soybean has excellent characteristics such as high protein content, strong stress resistance, and large reproduction coefficient. It is an important gene source for trait improvement and variety breeding of cultivated soybean, and is also a valuable resource for studying the origin, evolution, and classification of soybean. The distribution of wild soybeans in the world is very narrow, limited to the non-ari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00C05G3/00
CPCA01G22/00C05D9/00C05G3/00C05F11/00C05F11/04C05F11/08C05G1/00
Inventor 王栋卢新雄辛霞尹广鹍王效睦丁汉凤谷晓红张晓冬李润芳李湛
Owner SHANDONG CROP GERMPLASM CENT
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