Reproduction and regeneration method applied to genetic integrity analysis of Glycine soja Sieb.et Zucc.
A technology of wild soybeans and updated methods, applied in the direction of botany equipment and methods, applications, organic fertilizers, etc., can solve the problems of irregular emergence, long dormant period, low germination rate, etc., and achieve the promotion of germination and rooting, high reliability and Accuracy, the effect of improving seedling raising effect
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Embodiment 1
[0053] Embodiment 1 Apply the method of the present invention and the conventional method to carry out breeding renewal and genetic integrity analysis to wild soybean
[0054] (1) Test materials: wild soybean seeds from Laoshan Mountain were used as test materials.
[0055] (2) Test method:
[0056] 1. Method one: use the method provided by the invention to reproduce and update Laoshan wild soybeans. Proceed as follows:
[0057] (1) Reproduction and reproduction of Laoshan wild soybean parental treatment
[0058] ① Breeding site selection:
[0059] a. Breeding area: select the germplasm origin. Since the origin of wild soybeans in Laoshan is Laoshan District, Qingdao City, Shandong Province, a local farmer was found and the land was leased for reproduction and renewal, which can satisfy the growth and development of the reproduction and renewal materials and the expression of their traits.
[0060] b. Experimental site: flat terrain, uniform soil strength, regular shape, ...
Embodiment 2
[0149] Embodiment Two Wild Soybean Breaks Hard Solid Method Comparison
[0150] (1) Test materials and methods
[0151] 1. Test material
[0152] The test materials are mature annual wild soybean seeds, which were collected from Laoshan District, Qingdao City in 2014, that is, Laoshan wild soybean.
[0153] 2. Method
[0154] (1) Determination of seed moisture and thousand-grain weight
[0155] Refer to GB / T3543.4 for the inspection of crop seeds.
[0156] (2) Determination of seed hardness rate
[0157] Randomly pick 100 × 2 wild soybean seeds and soak them in water at room temperature for 24 hours, count the number of non-imbibed seeds, and calculate the firmness rate.
[0158] (3) The method of breaking the hard solid
[0159] 1. The method of the present invention: according to the following proportions: 6~7mg / L of thalassin and 20~30mg / L of lemongrass water extract are prepared into a solution with warm water at 35~50°C. Put wild soybeans into the solution for soak...
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