Manufacturing and application method of bioenzyme-process keratinization mask

A technology of biological enzyme method and production method, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of leading enterprises without representative significance, and achieve high safety effects

Inactive Publication Date: 2017-02-01
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current facial mask market, there is no domestic production and sales of facial masks that use purified keratinase biological exfolia

Method used

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Experimental program
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Embodiment Construction

[0019] The present invention is further described in detail below.

[0020] A method for making and using a bio-enzymatic exfoliating facial mask, characterized in that:

[0021] The casein material is pulverized, sterilized by high-pressure steam for 30 minutes, and then baked in an oven at 80°C for 12 hours to constant weight;

[0022] Keratinase-producing bacteria were inoculated in 100ml LB medium (Luria-Bertani medium) according to 1% inoculum amount, 160rpm / min (rotation speed / minute), and activated by shaking culture for 24 hours; then inoculated into 150ml LB ( Luria-Bertani medium) culture medium, 160 rpm, about 3-4 hours, shaking culture to logarithmic phase (OD6 00 0.4 ~ 0.6, OD: optical density) to obtain keratinase-producing bacterial inoculum;

[0023] 5 L fermenter to produce keratinase, liquid volume 3.5 L, 52.5 g of materials rich in keratinized protein such as pre-treated feathers, hair, keratinized skin or casein, K 2 HPO 4 4.9 g, KH 2 PO 4 2.45 g, N...

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PUM

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Abstract

The invention discloses a manufacturing and application method of a bioenzyme-process keratinization mask. The method comprises the following steps: pretreating bioenzyme-process mask raw materials, preparing keratinase, optimizing enzyme action conditions, combining the keratinase with a cotton linter mask, carrying out keratinase low-temperature preservation, and using the mask at room temperature. The keratin degrading bacterium Microbacterium sp. TSMC-99 is fully utilized to ferment and produce the keratinase, and the keratinase adsorbed on the mask catalyzes the degradation of partial keratinized tissues, thereby removing the keratinized scurf on the face, removing dead skin, moisturizing the face, enabling the skin to be clear and bright and performing the function of rejuvenating the skin. The bioenzyme-process keratinization mask is a biological high-tech product which has the advantages of no toxicity, no pollution, no side effect and high safety. The method has the advantages of mild production conditions, environment friendliness and low cost, and is simple to operate and easy for popularization. The technical indexes of the prepared mask satisfy the specifications in Light Industry Standard for masks QB/T 2872-2007.

Description

technical field [0001] The invention relates to a method for making and using a bio-enzymatic exfoliating facial mask. Background technique [0002] With the progress of the times and the development of the economy, more and more people begin to pay attention to the maintenance of personal skin quality. Among them, facial masks have been recognized by more and more people as cosmetics for cleaning, caring and nourishing the facial skin. Facial mask has become the fastest-growing market segment in my country's daily chemical industry. It ranked third in the cosmetics market in 2013 and 2014, accounting for 16.3% of the total market sales. In 2015, it reached 30 billion yuan, and the future market space will reach 200 billion yuan. Yuan. According to the latest report released by Kantar Worldpanel, facial masks contributed 41% of the sales increase to the skin care market in 2015. In 2015, 48% of urban households in China bought facial masks, and its development has surpassed...

Claims

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Application Information

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IPC IPC(8): A61K8/66A61K8/64A61K8/02A61Q19/00C12N9/52C12R1/01
CPCA61K8/0212A61K8/64A61K8/66A61K2800/85A61Q19/00C12N9/52
Inventor 史仁玖张显忠李艳玲
Owner TAISHAN MEDICAL UNIV
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