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Polypeptides for inhibiting proliferation of cancer cells, and uses thereof

A use, technology for gastric cancer, applied in the direction of antineoplastic drugs, drug combinations, etc.

Inactive Publication Date: 2017-02-15
北京京爱康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN100418574C records more than 20 kinds of peptides, which can specifically and firmly bind to the promoters of more than 40 downstream genes driven by the HIF-1α gene itself or its expressed transcription factors. Molecular pathways of cellular hypoxic responses to glycolytic and / or immune-inflammatory and / or angiogenic responses to chronically shut down advanced cancer cells or uncontrolled proliferation of chronic inflammatory cells under hypoxic conditions

Method used

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  • Polypeptides for inhibiting proliferation of cancer cells, and uses thereof
  • Polypeptides for inhibiting proliferation of cancer cells, and uses thereof
  • Polypeptides for inhibiting proliferation of cancer cells, and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] Peptide synthesis

[0028] Synthesize the polypeptides of sequences 1-8 in the sequence listing by solid-phase peptide synthesis (SPPS), including stepwise addition of amino acids protected with protecting groups to growing oligopeptide chains, which are anchored to chemically stable particles , so that the synthesized peptide can be separated from reagents and solvents by simple filtration. After the synthesis, the chain is cleaved from the carrier and purified.

[0029] Peptide efficacy test

[0030] Inhibitory effect on cancer cells

[0031] Cell Lines and Clinical Materials

[0032] Human colon cancer cell lines HCT116, DLD-1, rectal cancer cell lines SW1463, NCI-H630, liver cancer cell lines Hep-G2, lung cancer cell lines NCI-H460, gastric cancer cell lines MGC-803, breast cancer cell lines MCF-8 were all Commercially available, it is deposited at the American Type Culture Collection (Rockville, MD). All these cells were cultured in a monolayer in a suitable me...

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PUM

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Abstract

The present invention relates to polypeptides for inhibiting the proliferation of cancer cells, and uses thereof. According to the present invention, a series of polypeptides specifically bound to myc family genes are adopted as research objects to determine the treatment effects of the polypeptides on malignant tumors, particularly large intestine cancer, more particularly colon cancer, wherein the polypeptides are VKRRTHNVLERQRR (SEQ ID NO.1), RSTHNEMEKNRRA (SEQ ID NO.2), RSSHNELEKHRR (SEQ ID NO.3), KRAHHNALERKRR (SEQ ID NO.4), RSTHNELEKNRR (SEQ ID NO.5), NSLDPGLFVEST (SEQ ID NO.6), REVHNKLEKNRR (SEQ ID NO.7), and RRKEKSRDAARSRR (SEQ ID NO. 8). The present invention further provides uses of the polypeptides in preparation of drugs or pharmaceutical compositions for treatment of large intestine cancer, particularly colon cancer.

Description

technical field [0001] The present invention relates to the technical field of malignant tumor treatment. In particular, the present invention relates to the field of colorectal cancer treatment. More specifically, the present invention relates to the use of a polypeptide capable of specifically blocking specific gene cell proliferation pathways of human and mammalian cancer cells in the treatment of colorectal cancer, including rectal cancer and colon cancer. Background technique [0002] Colorectal cancer is the general term for colon cancer and rectal cancer. It refers to the malignant lesions of the large intestine mucosal epithelium under the action of various carcinogenic factors such as environment or genetics. It is a malignant tumor originating from the large intestine mucosal epithelium. First, it ranks third among malignant tumors in the world, and with the improvement of living standards and changes in dietary structure, the incidence of colorectal cancer contin...

Claims

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Application Information

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IPC IPC(8): A61K38/10A61P35/00
Inventor 谭樵夫
Owner 北京京爱康科技有限公司