Detection method of methyl jasmonate in plants based on microelectrode biosensing technology

A technology of methyl jasmonate and biosensors, applied in the direction of material electrochemical variables, etc., can solve the problems of inability to realize online detection of plant growth, large sample damage, and complicated operation, and achieve reliable detection results, small sample damage, and easy operation. simple effect

Active Publication Date: 2018-12-25
BEIJING RES CENT FOR INFORMATION TECH & AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the instruments used in these methods are complex in operation, high in cost, long in cycle, and highly destructive to samples, making it impossible to realize online detection of plant growth.

Method used

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  • Detection method of methyl jasmonate in plants based on microelectrode biosensing technology
  • Detection method of methyl jasmonate in plants based on microelectrode biosensing technology
  • Detection method of methyl jasmonate in plants based on microelectrode biosensing technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Microelectrode biosensor and its preparation method

[0024] In this embodiment, micro-electromechanical technology is used to prepare micro-electrodes on an insulating stainless steel substrate. The micro-electrodes are in the shape of a triangular pyramid with a total length of 5 cm and a tip portion of 5 mm in length. The cross-section of the other end is an equilateral triangle with a side length of 3mm; on each surface of the base, one end is provided with an element for connecting to an electrochemical workstation, and the other end is an electrode material for detection, and the element and the electrode material are connected by wires; the microelectrode has Three-electrode system, including Ag / AgCl reference electrode, platinum counter electrode and gold working electrode modified by platinum, graphene and perfluorosulfonic acid resin ( figure 1 and figure 2 ).

[0025] The gold working electrode modified by platinum, graphene and perfluorosulfonic...

Embodiment 2

[0029] Example 2 Real-time on-line detection of plant methyl jasmonate by microelectrode biosensor

[0030] 1. Subjects

[0031] The plant to be tested was the wheat variety 'Nongda 6812' grown by the Beijing Academy of Agriculture and Forestry Sciences, and the stems of two wheat seedlings were selected as test samples.

[0032] After cleaning, insert the microelectrode prepared in Example 1 into the stem of the sample to be tested, and start electrochemical detection.

[0033] 2. Experimental instruments and consumables

[0034] The experimental equipment was Shanghai Chenhua Electrochemical Workstation 760e.

[0035] 3. Experimental method

[0036] (1) Drawing of standard curve:

[0037] Before the detection, different concentrations of methyl jasmonate standard solutions were selected for differential pulse voltammetry scanning, and the working curve for microelectrode detection of methyl jasmonate was obtained according to the relationship between the concentration of...

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Abstract

The invention provides a method for detecting methyl jasmonate in plants on the basis of a microelectrode biosensing technique. The methyl jasmonate in the plants is detected on line through a microelectrode biosensor. The method has the characteristics of being high in accuracy, little in sample damage, simple to operate and convenient to carry, synchronous acquisition of information of the methyl jasmonate in the plants is realized in time dimension and space dimension, and technical support is provided for research of the plants. Compared with the traditional bioassay and chemical detection methods, the method has the advantages that sample pretreatment is simple, in-vitro detection, small damage and essential harm of a detected part are avoided, substantial damage to the plants is avoided, a detection result is reliable, and the sensitivity is high.

Description

technical field [0001] The invention relates to a microelectrode biosensing technology, in particular to a method for detecting methyl jasmonate in plants based on the microelectrode biosensing technology. Background technique [0002] Methyl jasmonate, a kind of growth regulating substance widely present in plants, has a variety of physiological effects, such as improving the resistance of seedlings to high and low temperature drought, and stimulating the synthesis of antifungal proteins to initiate or accelerate the defense response. Secondary metabolism, inhibition of growth and differentiation, promotion of rooting, maturation and senescence, etc. At present, due to the important physiological effects of methyl jasmonate and its relatively low content in plants, the detection of methyl jasmonate has attracted more and more attention. [0003] At present, the methods used to detect methyl jasmonate in plants are mainly chromatography, including gas chromatography-mass sp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30
CPCG01N27/30
Inventor 赵春江胡叶王晓冬李爱学何璐璐周航侯佩臣潘大宇宋鹏高权
Owner BEIJING RES CENT FOR INFORMATION TECH & AGRI
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