Method for detecting catecholamine in blood plasma by liquid chromatography tandem mass spectrometry

Abstract

The invention discloses a method for detecting catecholamine in blood plasma by liquid chromatography tandem mass spectrometry. The method comprises the following steps of preparing a standard product, separating by liquid chromatography, detecting by a mass spectrum, drawing a standard curve, and detecting the catecholamine in the blood plasma. The method has the beneficial effects that the catecholamine is detected by innovatively applying a high-flux liquid chromatography tandem triple quadrupole mass spectrograph; protein is precipitated by acetonitrile, dansyl chloride is used for performing derivative reaction, and the liquid chromatography tandem triple quadrupole mass spectrograph is used for detecting after extraction, so that the pretreatment steps are simple, the interference of a matrix in the blood plasma is effectively removed, and the specificity is good; after the derivatization of dansyl chloride, the detection sensitivity is effectively improved; the high-flux liquid chromatography tandem rod mass spectrograph is used for detecting, three types of catecholamine, including dopamine, epinephrine and norepinephrine, in the blood plasma can be quantitatively and accurately detected; the detection time is short, the flux is high, the detection sensitivity is high, the specificity is good, and the cost is low.

Description

technical field [0001] The invention relates to the technical field of catecholamine detection, in particular to a method for detecting catecholamines in blood plasma by using liquid chromatography tandem mass spectrometry. Background technique [0002] Catecholamine is a neurological substance containing catechol and amine groups, including epinephrine (epinephrine, E), norepinephrine (norepinephrine, NE), and dopamine (dopamine, DA), which are produced by the adrenal medulla and some sympathetic neurons. It is a very important neurotransmitter secreted by neuronal chromaffin cells, and it is also an important hormone substance. All three catecholamines are converted from tyrosine. Norepinephrine and epinephrine are both hormones secreted by the adrenal medulla and neuromediators of noradrenergic fibers in the sympathetic and central nervous systems. Noradrenaline is widely distributed in the central nervous system, and its content is relatively large, while that of epine...

AI Technical Summary

Problems solved by technology

[0003] At present, the commonly used catecholamine detection methods mainly include radioenzyme method, chemiluminescence method, fluorescence method and high performance liquid chromatography, etc. These detection methods mainly have the following problems: First, the pretreatment process of plasma samples is complicated and requires initial The amount of sample is large; second, the existing detection technology takes a long time, the experimental error is large, and the throughput is low; third, because epinephrine and methoxynorepinephrine have the same molecular weight and very similar structure, they cannot be detected by conventional methods. Different methods, poor specificity

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