A method and application of rapid detection of agarwood inducer transport law in agarwood-producing plants
An agarwood inducer and agarwood technology, which are applied in the biological field, can solve the problems of difficulty in determining the transmission distance and location of the agarwood inducer, time and energy consumption, pale color, etc., so as to reduce the death of the whole plant and save time and cost. , The effect of improving the aroma production rate
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Embodiment 1
[0044] Example 1 Rapid detection of the law of agarwood inducer delivery in the infusion method
[0045] (1) Infusion: Calculated by mass percentage, mix 1% methyl jasmonate and 1% ferrous sulfate at a ratio of 1:10 to prepare the agarwood inducer, then add 0.01g ferric chloride to the prepared 100ml agarwood inducer In, that is, the mass concentration of ferric chloride contained in the agarwood inducer is 0.01%.
[0046] Choose 3-year-old trees with a DBH of 8 cm, drill an infusion hole 5 cm deep perpendicular to the trunk at a distance of 45 cm from the ground, and introduce 100 ml of the agarwood inducer containing ferric chloride through the infusion hole through the infusion method. White wood incense tree body, and then seal the hole.
[0047] (2) Color development: After the infusion of the agarwood inducer is completed, the stems or branches at different distances from the infusion hole are cut off with a sharp hacksaw, and then freshly prepared acidic potassium ferr...
Embodiment 2
[0049] Example 2 Rapid detection of the conduction law of the agarwood inducer in the infusion method
[0050] (1) Infusion: Add 0.5g ferrous chloride to 100ml of agarwood inducer, the preparation of this agarwood inducer is calculated by mass percentage: 0.1% methyl jasmonate, 0.5% sodium chloride and 0.3% phenylethanol solution Mixed according to 1:5:3.
[0051] Select a 5-year-old tree with a DBH of 10cm, and drill an infusion hole with a depth of 0.6cm and a depth of 7cm perpendicular to the trunk at a distance of 100cm from the ground, and prepare 100ml of ferrous chloride containing 0.5% The agarwood inducer is slowly injected into the tree through the infusion hole.
[0052] (2) Color development: After the infusion of the agarwood inducer is finished, the stems or branches at different distances from the infusion hole are cut off with a sharp hacksaw, and the freshly prepared 2% potassium ferricyanide solution is dripped on the section, as figure 2 As shown, the pos...
Embodiment 3
[0053] Example 3 Rapid detection of agarwood inducer transport law in high-pressure injection method
[0054] (1) Infusion: add 0.2g ferric chloride to 100ml agarwood inducer, the preparation of this agarwood inducer is calculated by mass percentage: 0.04% gibberellin and 1% sodium bisulfite solution are mixed at 1:25 made by mixing.
[0055] Choose a 5-year-old tree with a DBH of 12cm, and drill a 7cm infusion hole at a distance of 150cm from the ground. Use 6HZ-W1020 type high-pressure trunk injection machine to inject 100ml of agarwood inducer containing 0.2% ferric chloride into the trunk of Akiras sinensis through the infusion hole.
[0056] (2) Color development: After the injection is completed, cut off the stems or branches at different distances from the infusion hole with a sharp hacksaw, and freshly prepared acid potassium ferrocyanide (4% potassium ferrocyanide and 4% hydrochloric acid in equal amounts), drop it on the cross-section, the position where the agarwo...
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