A kind of enriched fraction of glutarum phenolic acid and its application in the preparation of antitumor drugs

A technology of anti-tumor drugs and phenolic acids, which can be used in the fields of anti-tumor drugs, drug combinations, sugar derivatives preparation, etc., to achieve a good effect of inhibiting proliferation

Active Publication Date: 2020-03-31
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the inhibition of Aurora kinase A / B by the phenolic acids in Gujingcao

Method used

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  • A kind of enriched fraction of glutarum phenolic acid and its application in the preparation of antitumor drugs
  • A kind of enriched fraction of glutarum phenolic acid and its application in the preparation of antitumor drugs
  • A kind of enriched fraction of glutarum phenolic acid and its application in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0022] Example 2: In vitro anti-tumor mechanism study of phenolic acids enriched fractions in Herba Gujingensis.

[0023] (1) Instruments and reagents: RPMI Medium 1640 culture medium and fetal bovine serum were purchased from Hyclone Company of the United States. Trypan blue, Tris-base, acrylamide, methylene bisacrylamide, sodium dodecyl sulfate (SDS), tetrahexylhexamethylene diamine (TEMED), sodium persulfate, dimethyl diphenyl tetra Triazolium bromide (MTT) was purchased from Amresco, USA. Glycine and propidium iodide (PI) were purchased from Sigma, USA. Annexin V-FITC assay kit was purchased from KGI Biotech. CKX31 inverted microscope (Olympus, Philippines); constant temperature CO2 incubator (Thermo, USA); 5810R desktop high-speed low-temperature centrifuge (Eppendorf, Germany); automatic microplate reader (Biotek Group, USA).

[0024] (2) Cell culture: K562 was cultured in 1640 medium (containing 10% fetal bovine serum), 37°C, containing 5% CO 2 cultured in a humidif...

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Abstract

The invention relates to a Flos Eriocauli phenolic acids enrichment fraction and application in the preparation of an antitumor drug and belongs to the field of pharmacy, specifically relates to a Flos Eriocauli phenolic acids enrichment fraction, a preparation method and application in the preparation of a medicine with Aurora kinase A / B inhibiting effect. By a cytobiological method, proliferation suppressing activity and mechanism of human leukemia cells (K562) by phenolic acids enrichment fraction in Flos Eriocauli are measured. Results show that phenolic acids fraction in Flos Eriocauli achieves the effect of inhibiting cell proliferation by inhibiting activity of Aurora kinase A / B. Flos Eriocauli, used as a edible and medicinal Chinese herbal medicine, is expected to be developed into an efficient low-toxicity antitumor drug which is applied in clinic.

Description

Technical field: [0001] The invention belongs to the field of pharmacy, and in particular relates to an enriched fraction of glutarum phenolic acids, its preparation method and its application in the preparation of drugs with Aurora kinase A / B inhibitory effect. Background technique: [0002] The Aurora kinase family is an important class of serine / threonine kinases in the cell mitosis regulatory network, including three family members of Aurora kinase A, B, and C. In cell mitosis, Aurora kinase is involved in multiple processes such as centrosome maturation and separation, chromosome condensation, spindle assembly and maintenance, chromosome segregation, and cytokinesis. It was discovered in 1995 and first observed in human tumor tissues in 1998. After expression, Aurora Kinase has gradually become a research hotspot in the academic and oncological circles to synergistically and efficiently inhibit the growth of malignant tumor cells. [0003] The expression levels of A an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H13/08C07H1/08C07D311/30C07D311/40C07C51/42C07C65/03A61K31/7048A61K31/7024A61K31/352A61K31/192A61P35/00
Inventor 赵庆春范艳华周志鹏
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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