A kind of bacillus subtilis and application thereof

A technology of Bacillus subtilis, preservation number, applied in the application, bacteria, microorganisms and other directions, can solve the problem of reducing the activity of microbial feed additives

Active Publication Date: 2019-11-15
福建洛东生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems of microbial feed additives are the selection of good strains and the reduction of the activity of microbial feed additives due to the inactivation of strains

Method used

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  • A kind of bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 chicken farm test

[0023] In this experiment, 480 31-week-old Hai-Line brown layer hens were randomly divided into two groups, 240 in the control group and 240 in the test group, and the test period was 21 days. Test diet: 61.5% corn, 23.4% soybean meal, 2% wheat bran, 8.1% stone powder, 5% compound premix, no health-care antibiotics were fed during the test.

[0024] The test group added 0.1‰ microbial feed additives to the feed during the test period. The microbial feed additive consists of 1.0% Bacillus subtilis (the preservation number is CCTCC M2016442, and the number of viable bacteria is 1.0×10 9 cfu / g) and 99.0% glucose are evenly mixed. The control group did not add bacteria, and other feeding conditions were the same.

[0025] The test results are as follows:

[0026] project egg weight(g) Egg production rate (%) Broken egg rate (%) Egg rate (%) Daily Consumption (g) feed-to-egg ratio control group 63.4±1.25 87.3±4...

Embodiment 2

[0028] Embodiment 2 microbial feed additive

[0029] 1% preservation number is the Bacillus subtilis of CCTCC M2016442 (viable bacteria number is 1.0×10 9 cfu / g), 0.1% of the preservation number is CCTCC M 2016440 Bacillus coagulans (the number of live bacteria is 1.0×10 8 cfu / g), 1% Saccharomyces cerevisiae (from Angel Yeast Co., Ltd., the number of viable bacteria is 5.0×10 7 CFU / g), mixed with 97.9% glucose evenly.

Embodiment 3

[0030] Embodiment 3 fattening pig feeding test

[0031] In a pig farm, 6 pens of fattening pigs were randomly selected as test objects, with about 70 pigs in each pen. The 1st, 2nd, and 3rd pens were the control group, and the 4th, 5th, and 6th pens were the test groups. During the test, 200g of microbial additives were added per ton of feed.

[0032] The test group used the microbial feed additive formulated in Example 2.

[0033] The control group uses 0.1% of Bacillus coagulans with the preservation number CCTCC M 2016440 (the number of viable bacteria is 1.0×10 8 cfu / g), 1% Saccharomyces cerevisiae (from Angel Yeast Co., Ltd., the number of viable bacteria is 5.0×10 7 CFU / g), and a microbial additive made by mixing 98.9% glucose.

[0034] The test time was 30 days in total, during which the aureomycin, acidulant and sweetener for health care were removed.

[0035]

[0036] As can be seen from the table in Example 3, the fattening pigs were fed for 30 days, and the a...

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Abstract

The invention relates to a bacillus subtilis preserved in CCTCC with the preservation number of CCTCC M2016442 and its application in poultry digestion. The nattokinase produced by the bacillus subtilis can improve the blood circulation of poultry and livestock, enhance the immunity of poultry and livestock, and at the same time have a strong ability to produce digestive enzymes, which can promote the digestion of feed by poultry and livestock. This product is used in the field of microbial feed additives, which can improve the intestinal flora of livestock and poultry that eat feed, improve the digestion and immunity of livestock and poultry, enhance the digestion and transformation of feed for livestock and poultry, and promote animal growth , significantly improve the feed utilization rate of livestock and poultry, has obvious economic value.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a bacillus subtilis and its preparation and application. Background technique [0002] Bacillus subtilis is a species of Bacillus. A single cell is 0.7-0.8×2-3 microns, evenly colored. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center of the bacterium or slightly biased, the bacterium does not expand after spore formation. The surface of the colony is rough and opaque, stained white or yellowish, and often forms wrinkles when growing in liquid medium. Aerobic bacteria can use protein, various sugars and starch to decompose tryptophan to form indole. It is widely used in genetic research, and the synthesis pathway and regulation mechanism of purine nucleotides in this bacterium are relatively clear. Widely distributed in soil and decaying organic matter, it is eas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/30A23K50/75C12R1/125
CPCC12N1/205C12R2001/125
Inventor 刘明刚李坤卢祯鑫
Owner 福建洛东生物技术有限公司
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