A kind of Bacillus subtilis and its preparation and application

A technology of Bacillus subtilis and its preparation, which is applied in the field of Bacillus subtilis and its preparation and application, and can solve the problems of reduced activity of microbial feed additives, etc.

Active Publication Date: 2020-07-17
福建洛东生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems of microbial feed additives are the selection of good strains and the reduction of the activity of microbial feed additives due to the inactivation of strains

Method used

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  • A kind of Bacillus subtilis and its preparation and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 organic fertilizer fermentation test

[0035] There were 2 control groups and 1 test group in this experiment. Each group composts 3 groups of organic fertilizers according to the weight ratio of 85% pig manure and 15% rice straw. Among them, the control group 2 added 0.2% by weight of live bacteria in the compost raw materials, and the content was 1.0×10 10 The commercially available Bacillus subtilis of CFU / g (preserved in China General Microorganism Culture Collection and Management Center, the preservation number is CGMCC1.3358); the preservation number of the test group added 0.2% by weight in the compost raw material is CCTCC M2016441, the live bacteria content 1.0×10 10 CFU / g of Bacillus subtilis. The 3 groups of organic fertilizers are mixed and piled up, and turned over every other day.

[0036] The fermentation time of control group 1 is 28 days, wherein the 3rd to 12th days are the high temperature period, and the temperature in the high temp...

Embodiment 2

[0043] Embodiment 2 chicken farm test

[0044] In this experiment, 300 20-week-old Hai-Line brown layer hens were randomly divided into a control group and a test group of 150 each, and the test period was 24 days. Test diet: 61.5% corn, 23.4% soybean meal, 2% wheat bran, 8.1% stone powder, 5% compound premix, no health-care antibiotics were fed during the test.

[0045] The test group added 0.1‰ microbial feed additives by weight to the feed during the test period. The microbial feed additive consists of 1.0% by weight of Bacillus subtilis (the number of live bacteria is 1.0 × 10 11 cfu / g) mixed with 99.0% by weight glucose.

[0046] In the control group, the same amount of glucose was added to the feed. Other feeding conditions were the same.

[0047] The test results are as follows:

[0048] project Single egg weight (g) Egg production rate (%) Broken egg rate (%) Egg rate (%) Daily Consumption (g) feed-to-egg ratio control group 63.4±1.25 ...

Embodiment 3

[0051] Embodiment 3 nursing pig feeding test

[0052] In this experiment, two pens of nursery pigs with the same initial weight and half male and female were randomly selected in the nursery of the pig farm for the test. The number of each pen was 18. One of the pens was set as the control group and the other as the test group. The test group added 0.1‰ of Bacillus subtilis with the preservation number of CCTCC M2016441 to the common diet, and the control group was fed the normal diet without adding the strain.

[0053] The weight of each pig before and after feeding was recorded, and the feeding process was completed by the same breeder.

[0054]

[0055] The test results showed that after 31 days of feeding, the average weight gain of the test group was 1.2kg higher than that of the control group, and the feed-to-meat ratio decreased by more than 0.3.

[0056] The test results show that adding Bacillus subtilis with the preservation number CCTCC M2016441 to the feed in p...

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Abstract

The invention relates to a bacillus subtilis preserved in CCTCC with the preservation number of CCTCC M2016441 and its application in promoting protein decomposition. The protease produced by the bacillus subtilis has strong activity and can strongly promote the decomposition of protein substances. The Bacillus subtilis and its solvent or the composition comprising the Bacillus subtilis can be applied in various fields. In organic fertilizer fermentation, it can solve the problem of ammonia volatilization in organic fertilizer fermentation, reduce nitrogen loss and protect fertilizer efficiency. In the application of deodorization and antibacterial in farms, it can reduce the odor. In the application of harmless treatment of livestock and poultry carcasses, the carcasses can be quickly and harmlessly disposed of. In the application of biological fermentation bed, it can achieve pollution-free treatment of animal manure, and can also produce organic fertilizer. In the feed composition, it can improve the intestinal flora of livestock and poultry that eat feed, improve the digestibility and immunity of livestock and poultry, enhance the digestion and transformation ability of livestock and poultry that eat feed, and at the same time deodorize and relieve animal Barn or farm odor.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a bacillus subtilis and its preparation and application. Background technique [0002] Bacillus subtilis is a species of Bacillus. A single cell is 0.7-0.8×2-3 microns, evenly colored. Uncapsulated, with periosteum flagella, able to move. Gram-positive bacteria, spores 0.6-0.9×1.0-1.5 microns, elliptical to columnar, located in the center of the bacterium or slightly biased, the bacterium does not expand after spore formation. The surface of the colony is rough and opaque, stained white or yellowish, and often forms wrinkles when growing in liquid medium. Aerobic bacteria can use protein, various sugars and starch to decompose tryptophan to form indole. It is widely used in genetic research, and the synthesis pathway and regulation mechanism of purine nucleotides in this bacterium are relatively clear. Widely distributed in soil and decaying organic matter, it is eas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00A61L9/013A01N63/22C12R1/125A61L101/52
CPCA01N63/00A61L9/013A61L2209/21C05F3/00C05F17/00C12N1/205C12R2001/125C05F11/00C05F11/08C05F5/002Y02W30/40Y02P60/21
Inventor 李坤刘明刚卢祯鑫
Owner 福建洛东生物技术有限公司
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